2013 Fiscal Year Final Research Report
Pathophysiological analysis for the ischemic brain damage using in vitro blood-brain barrier system
| Project/Area Number |
23659460
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MINAMI Masabumi 北海道大学, 大学院薬学研究院, 教授 (20243040)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 血液脳関門 / 骨髄幹細胞 / 脳実質浸潤 / セルマイグレーション / リアルタイムイメージング |
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| Research Abstract |
To study transmigration of mesenchymal stem cells (MSCs) across the blood-brain barrier (BBB), we developed an in vitro BBB system consisting of rat brain microvascular endothelial cells (BMECs). Time-lapse imaging using this system revealed that MSCs transmigrated across the BMEC monolayer through transiently formed intercellular gaps between the BMECs. In addition, we developed an in vitro imaging system for simultaneous analysis in real time between claudin-5, a tight junction protein, and intracellular calcium dynamics in BMECs during MSC transmigration.
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