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2012 Fiscal Year Final Research Report

Elucidation of the target genes for the treatment of lipid disorder by gene-trap method in human haploid cells

Research Project

  • PDF
Project/Area Number 23659469
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Metabolomics
Research InstitutionKyoto University

Principal Investigator

ONO Koh  京都大学, 医学研究科, 講師 (00359275)

Project Period (FY) 2011 – 2012
Keywords遺伝子トラップ / ハプロイド / マイクロ RNA
Research Abstract

We have developed gene-trap method and applied it to adipocyte differentiation. However, mammalian cells are diploid and only such genes which have haploid insufficiency can be obtained by this method. This time we utilized P1-55 cells, which is a human haploid cell line, and enabled to screen all genes by gene-trap method.

  • Research Products

    (33 results)

All 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (15 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Metastasis-associated protein, S100A4 mediates cardiac fibrosis potentially through the modulation of p53 in cardiac fibroblasts2013

    • Author(s)
      Tamaki Y, Iwanaga Y, Niizuma S, Kawashima T, Kato T, Inuzuka Y, Horie T, Morooka H, Takase T, Akahashi Y, Kobuke K, Ono K, Shioi T, Sheikh SP, Ambartsumian N, Lukanidin E, Koshimizu TA, Miyazaki S, Kimura T.
    • Journal Title

      J Mol Cell Cardiol

      Volume: 57 Pages: 72-81

    • Peer Reviewed
  • [Journal Article] MicroRNA-33-deficiency reduces the progression of atherosclerotic plaque in apoE-/- mice2012

    • Author(s)
      Horie T, Baba O, Kuwabara Y, Chujo Y, Watanabe S, Kinoshita M, Horiguchi M, Nakamura T, Chonabayashi K, Hishizawa M, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, Ono K.
    • Journal Title

      J Am Heart Assoc

      Volume: 1 Pages: e003376

    • Peer Reviewed
  • [Journal Article] Current concept of reverse cholesterol transport and novel strategy for atheroprotection.2012

    • Author(s)
      Ono K
    • Journal Title

      J Cardiol.

      Volume: 60 Pages: 339-43

    • Peer Reviewed
  • [Journal Article] Highly Purified Eicosapentaenoic Acid Increases Interleukin-10 Levels of Peripheral Blood Monocytes in Obese Patients With Dyslipidemia2012

    • Author(s)
      Satoh-Asahara N, Shimatsu A, Sasaki Y, Nakaoka H, Himeno A, Tochiya M, Kono S, Takaya T, Ono K, Wada H, Suganami T, Hasegawa K, Ogawa Y
    • Journal Title

      Diabetes Care

      Volume: 35 Pages: 2631-9

    • Peer Reviewed
  • [Journal Article] MicroRNA 26b Encoded by the Intron of Small CTD Phosphatase (SCP) 1 Has an Antagonistic Effect on its Host Gene.2012

    • Author(s)
      Sowa N, Horie T, Kuwabara Y, Baba O, Watanabe S, Nishi H, Kinoshita M, Takanabe-Mori R, Wada H, Shimatsu A, Hasegawa K, Kimura T, and Ono K
    • Journal Title

      Journal of Cellular Biochem

      Volume: 113 Pages: 3455-65

    • Peer Reviewed
  • [Journal Article] α1-Antitrypsin-low-density-lipoprotein Serves as a Marker of Smoking-specific Oxidative Stress2012

    • Author(s)
      Wada H, Ura S, Satoh-Asahara N, Kitaoka S, Mashiba S, Akao M, Abe M, Ono K, Morimoto T, Fujita M, Shimatsu A, Takahashi Y, Hasegawa K.
    • Journal Title

      J Atheroscler Thromb

      Volume: 19 Pages: 47-58

    • Peer Reviewed
  • [Journal Article] Increased microRNA-1 and microRNA-133a Levels in Serum of Patients with Cardiovascular Disease Indicate the Existence of Myocardial Damage2011

    • Author(s)
      Kuwabara Y, Ono K, Horie T, Nishi H, Nagao K, Kinoshita M, Watanabe S, Baba O, Kojima Y, Shizuta S, Imai M, Tamura T, Kita T, Kimura T.
    • Journal Title

      Circulation: Cardiovascular Genetics

      Volume: 4 Pages: 446-454

    • Peer Reviewed
  • [Journal Article] MicroRNA links obesity and impaired glucose metabolism2011

    • Author(s)
      Ono K
    • Journal Title

      Cell Res

      Volume: 21 Pages: 864-6

    • Peer Reviewed
  • [Journal Article] MicroRNAs and Cardiovascular Diseases2011

    • Author(s)
      Ono K, Kuwabara Y, Han J
    • Journal Title

      FEBS J

      Volume: 278 Pages: 1619-33

    • Peer Reviewed
  • [Journal Article] MicroRNA-27a regulates beta cardiac myosin heavy chain gene expression by targeting thyroid hormone receptor beta 1 in neonatal rat ventricular myocytes2011

    • Author(s)
      Nishi H, Ono K, Horie T, Nagao K, Kinoshita M, Kuwabara Y, Watanabe S, Takaya T, Tamaki Y, Takanabe-Mori R, Wada H, Hasegawa K, Iwanaga Y, Kawamura T, Kita T, Kimura T
    • Journal Title

      Mol Cell Biol

      Volume: 31 Pages: 44-45

    • Peer Reviewed
  • [Journal Article] miRNA-33 は生体内でコレステロールを制御する2011

    • Author(s)
      尾野 亘、堀江貴裕、木村 剛、北 徹
    • Journal Title

      実験医学

      Volume: 29 Pages: 575-578

  • [Journal Article] マイクロ RNA と循環器疾患2011

    • Author(s)
      尾野 亘
    • Journal Title

      最新医学

      Volume: 66 Pages: 2580-2586

  • [Presentation] MicroRNA 33 Deficiency in Leukocytes Reduced the Lipid Accumulation in Atherosclerotic Plaque in ApoE Knockout Mice2012

    • Author(s)
      Baba O, Horie T, Kuwabara Y, Izuhara M, Usami S, Nakao T, Nishiga M, Nishino T, Sowa N, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, and Ono K
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Los Angeles Convention Center Los Angeles(U.S.A.)
    • Year and Date
      20121103-07
  • [Presentation] MicroRNA-33 Deficiency Reduced Atherosclerosis Formation In Vivo2012

    • Author(s)
      Horie T, Baba O, Ono K, Kuwabara Y, Hasegawa K, Kume N, Yokode M, Kita T, and Kimura T
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Los Angeles Convention CenterLos Angeles(U.S.A.)
    • Year and Date
      20121103-07
  • [Presentation] MicroRNA-33-deficiency reduces the progression of atherosclerotic plaque in apoE-/- mice2012

    • Author(s)
      Ono K
    • Organizer
      Cold spring harbor laboratory meeting. Regulatory and non-coding RNA
    • Place of Presentation
      NY, U.S.A
    • Year and Date
      20120828-0901
  • [Presentation] MicroRNA 33 Deficiency Reduces Atherosclerotic Plaque progression in ApoE Knockout Mice2012

    • Author(s)
      Baba O, Horie T, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, and Ono K.
    • Organizer
      European Society of Cardiology Congress
    • Place of Presentation
      Munich(Germany)
    • Year and Date
      20120825-29
  • [Presentation] Interaction of S100A4 with p53 in fibroblasts modulates cardiac fibrosis through two distinct mechanisms.2012

    • Author(s)
      Tamaki Y, Iwanaga Y, Ono K, Shioi T, Sheikh SP, Ambartsumian N, Lukanidin E, Koshimizu T, Miyazaki S, Kimura T
    • Organizer
      European Society of Cardiology Congress
    • Place of Presentation
      Munich(Germany)
    • Year and Date
      20120825-29
  • [Presentation] Symposium 6 (SY6) (HF) Diagnosis of Heart Failure Using Genetic Biomarker Increased MicroRNA-1 and MicroRNA-133a Levels in Serum of Patients with Cardiovascular Disease Indicate the Existence of Myocardial Damage2012

    • Author(s)
      Ono K
    • Organizer
      第76回日本循環器学会
    • Place of Presentation
      福岡
    • Year and Date
      20120318-20
  • [Presentation] MicroRNA-33 regulates atherosclerosis formation2012

    • Author(s)
      Horie T, Baba O, Ono K, Watanabe S, Nishi H, Kinoshita M, Kuwabara Y, Chujo Y, Sowa N, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T.
    • Organizer
      第76回日本循環器学会
    • Place of Presentation
      福岡
    • Year and Date
      20120318-20
  • [Presentation] Circulating microRNA derived from damaged cardiomyocytes can regulate the gene expression in recipient cells.2012

    • Author(s)
      Kuwabara Y, Ono K, Horie T, Nishi H, Watanabe S, Kinoshita M, Baba O, Chujo Y, Kita T, Kimura T
    • Organizer
      第76回日本循環器学会
    • Place of Presentation
      福岡
    • Year and Date
      20120318-20
  • [Presentation] MicroRNA 26b encoded by the intron of SCP1 has an antagonistic on its host gene and cardiac hypertrophy2012

    • Author(s)
      Sowa N, Ono K, Horie T, Hasegawa K, Kita T, Kimuta T
    • Organizer
      第76回日本循環器学会
    • Place of Presentation
      福岡
    • Year and Date
      20120318-20
  • [Presentation] Symposium 6 (Insulin resistance) MicroRNA regulation of lipid homeostasis.2012

    • Author(s)
      Ono K
    • Organizer
      25th Spring Congress of Korean Diabetes Association
    • Place of Presentation
      Hotel Inter-Burgo Exco, Daegu(Korea)
    • Year and Date
      2012-05-11
  • [Presentation] MicroRNA 26b encoded by the introns of small CTD Phosphatases (SCPs) 1 has an antagonistic effect on its host gene and suppresses cardiac hypertrophy2011

    • Author(s)
      Sowa N, Ono K, Horie T, Hasegawa K, Kimura T
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Orland(U.S.A.)
    • Year and Date
      20111112-16
  • [Presentation] MicroRNA-1 and microRNA-133a elevations in serum of patients with cardiovascular disease indicate the existence of myocardial damage.2011

    • Author(s)
      Kuwabara Y, Ono K, Horie T, Nishi H, Watanabe S, Kinoshita M, Baba O, Chujo Y, Nagao K, Kojima Y, Kita T, Kimura T
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Orlando(U.S.A.)
    • Year and Date
      20111112-16
  • [Presentation] Soluble vascular endothelial growth factor receptor-1 serves as a marker of cardiovascular risk in chronic kidney disease patients.2011

    • Author(s)
      Wada H, Ura S, Masunaga N, Akao M, Abe M, Funatsu J, Nakajima Y, Kanasaki M, Nakano T, Ono K, Morimoto T, Shimatsu A, Hasegawa K
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Orlando(U.S.A.)
    • Year and Date
      20111112-16
  • [Presentation] Plenary session. MicroRNA-33 Encoded by an Intron of Srebp2 Cooperates to Control HDL in Vivo2011

    • Author(s)
      Ono-K.
    • Organizer
      第75回日本循環器学会
    • Place of Presentation
      横浜市
    • Year and Date
      20110803-04
  • [Presentation] MicroRNA-33 Encoded by an Intron of Srebp2 Regulates HDL in vivo.2011

    • Author(s)
      Yeungnam University, Daegu(Korea) Koh Ono
    • Organizer
      2011AVDRC International Symposium, Intervention of Vascular Aging
    • Place of Presentation
      College of Medicine, Yeungnam University, Daegu(Korea)
    • Year and Date
      2011-09-22
  • [Book] 細胞工学 疾患エクソソーム2013

    • Author(s)
      尾野 亘
    • Total Pages
      27-31
    • Publisher
      秀潤社
  • [Book] Annual Review 糖尿病・代謝・内分泌2012

    • Author(s)
      尾野 亘
    • Total Pages
      80-85
    • Publisher
      中外医学社
  • [Book] Roles of MicroRNAs and Myocardial Cell Differentiation2012

    • Author(s)
      Tomohide Takaya
    • Total Pages
      139-152
    • Publisher
      Burlington: Academic Press
  • [Book] Annual Review 糖尿病・代謝・内分泌20122011

    • Author(s)
      尾野 亘
    • Total Pages
      240
    • Publisher
      中外医学社
  • [Remarks]

    • URL

      http://kyoto-u-cardio.jp/kisokenkyu/metabolic/

  • [Patent(Industrial Property Rights)] 大動脈瘤および大血管の炎症抑制治療製材2012

    • Inventor(s)
      尾野 亘
    • Industrial Property Rights Holder
      尾野 亘
    • Industrial Property Number
      61/720, 570
    • Filing Date
      2012-10-31

URL: 

Published: 2014-08-29   Modified: 2014-10-07  

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