Elucidation of energy metabolic pathways in hormone-dependent cancers by utilizing cellular and in vivo imaging analyses

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659479
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Saitama Medical University
• Principal Investigator: HORIE Kuniko 埼玉医科大学, 医学部, 准教授 (90261982)
• Co-Investigator(Kenkyū-buntansha): IKEDA Kazuhiro 埼玉医科大学, 医学部, 講師 (30343461)
• Co-Investigator(Renkei-kenkyūsha): INOUE Satoshi 東京大学, 医学部, 特任教授 (40251251)
• Project Period (FY): 2011 – 2012
• Keywords: 性ホルモン / がん / イメージング / エネルギー代謝

Research Abstract

In order to reveal characterize metabolic pathways involved in the proliferation and progression of hormone-dependent cancers such as breast and prostate cancers, cell models of hormone-naive and hormone-resistant cancer cellsexpressing fluorescent reporters were generated. A microscopic imaging system for the observation of these cancer cells was developed in response to environmental alterationsby monitoring reporter fluorescence. Inoculation of cancer cells stably expressing luciferase reporter genes into nude mice was performed and the dynamics of cancer cell proliferation and metastasis were investigated based on in vivo imaging analysis. Expression and transcriptional regulation of metabolic genes in hormone-dependent cancer cells were analyzed molecular biologically. Taken together, the present study identified novel molecular targets that can be applied to the diagnosis, therapy, and prevention of hormone-dependent cancers.

Research Products

• [Journal Article] Genome-wide integrated analyses of androgen receptor signaling in prostate cancer based on high-throughput technology (2013)
  • Author(s): Horie-Inoue K, Inoue S
  • Journal Title: Curr Drug Targets Volume: 14(4) Pages: 472-480
  • DOI: DOI:10.2174/1389450111314040008.
  • Peer Reviewed
• [Journal Article] Association of double-positive FOXA1 and FOXP1 immunoreactivities with favorable prognosis of tamoxifen-treated breast cancer patients. (2012)
  • Author(s): Ijichi N, Shigekawa T, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S.
  • Journal Title: Horm Cancer Volume: 3(4) Pages: 147-159
  • DOI: DOI:10.1007/s12672-012-0111-0
  • Peer Reviewed
• [Journal Article] TACC2 is an androgen-responsive cell cycle regulator promoting androgen-mediated and castration-resistant growth of prostate cancer. (2012)
  • Author(s): Takayama K, Horie-Inoue K, Suzuki T, Urano T, Ikeda K, Fujimura T, Takahashi S, Homma Y, Ouchi Y, Inoue S.
  • Journal Title: Mol Endocrinol. Volume: 26(5) Pages: 748-761
  • DOI: DOI:10.1210/me.2011-1242
  • Peer Reviewed
• [Journal Article] Forkhead box transcription factor, forkhead box A1, shows negative association with lymph node status in endometrial cancer, and represses cell proliferation and migration of endometrial cancer cells (2012)
  • Author(s): Abe Y, Ijichi N, Ikeda K, Kayano H, Horie-Inoue K, Takeda S, Inoue S
  • Journal Title: Cancer Sci Volume: 103(4) Pages: 806-812
  • DOI: DOI:10.1111/j.1349-7006.2012.02201.x.
  • Peer Reviewed
• [Journal Article] FOXP1, an estrogen-inducible transcription factor, modulates cell proliferation in breast cancer cells and 5-year recurrence-free survival of patients with tamoxifen-treated breast cancer (2011)
  • Author(s): Shigekawa T, Ijichi N, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S
  • Journal Title: Horm Cancer Volume: 2(5) Pages: 286-297.
  • DOI: DOI:10.1007/s12672-011-0082-6
  • Peer Reviewed
• [Presentation] Systemic screening of microRNAs nad their targets for breast cancer cells using a comprehensive lentiviral microRNA library and microarrays. (2012)
  • Author(s): Horie-Inoue K, Yamaga R, Okamoto K, Ikeda K, Inoue S.
  • Organizer: Keystone Symposia, Non-coding RNAs
  • Place of Presentation: Snowbird, Utah, USA.
  • Year and Date: 20120332-0405
• [Presentation] Cytochrome c oxidase subunit 7a related polypeptide COX7RP is an estrogen target gene that plays a role in the OXPHOS pathway. (2011)
  • Author(s): Ikeda K, Horie-Inoue K, Inoue S
  • Organizer: 5th Biennial Meeting of Society for Free Radical Research-Asia (SFRR-Asia), 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM), 11th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit)
  • Place of Presentation: 鹿児島市
  • Year and Date: 20110831-0904
• [Presentation] Identification of androgen-regulated cytochrome coxidase-related genes in hormone-sensitive prostate cancer cells through RNA-sequencing and systemic chromatin immunoprecipitation analysis (2011)
  • Author(s): Horie-Inoue K, Takayama K, Ikeda K, Suzuki Y, Inoue S
  • Organizer: Mitochondrial Assembly & Dynamics in Health, Disease & Aging, FASEB Summer Research Conferences
  • Place of Presentation: Steamboat Springs, Colorado, USA.
  • Year and Date: 20110717-22
• [Remarks]
  • URL: http://www.saitama-med.ac.jp/genome/Div02_GRST/index.html