2012 Fiscal Year Final Research Report
The analysis of epigenetic changes in GH-IGF-1 axis of small for gestational age rats
| Project/Area Number |
23659480
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Takahiro 日本医科大学, 医学部, 准教授 (40366654)
OHATA Hisayuki 日本医科大学, 医学部, 講師 (80256924)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 低出生体重 / 母胎低栄養 / 成長ホルモン受容体 / マイクロ RNA / DNA メチル化 |
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| Research Abstract |
The hormonal factors involved in catch-up growth in the small for gestational age (SGA) are unknown. To reveal the mechanism underlying growth failure in SGA, we analyzed growth hormone (GH)/ insulin-like growth factor (IGF)-1 axis in a rat model. In SGA and non-catch up growth (SGA-NCG) rats, hepatic growth hormone receptor (GHR) expression was reduced. We revealed that the reduction of GHR expression is caused by elevation of miR-322 expression in the liver of SGA-NCG rats. We further found that DNA methylation in the miR-322 coding region is reduced in the liver of SGA-NCG rats and thatthese changes in the expression of GHR and miR-322 are trans-generational.
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