2012 Fiscal Year Final Research Report
Production and analysis of Adamts13-Bac-TRAP mice that express fluorescent protein EGFP driven by Adamts13 promoter
| Project/Area Number |
23659494
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MIYATA Toshiyuki 独立行政法人国立循環器病研究センター, 研究所, 部長 (90183970)
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| Co-Investigator(Kenkyū-buntansha) |
BANNO Fumiaki 独立行政法人国立循環器病研究センター, 研究所, 研究員 (00373514)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 血栓性血小板減少性紫斑病 / ADAMTS13 / 抗血栓因子 / 肝星細胞 / トランスジェニックマウス / Bac-TRAP |
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| Research Abstract |
We developed novel BAC transgenic mice with cDNA encoding a fusion protein of a fluorescent protein EGFP and a ribosomal protein L10a under control of the hepatic stellate cell-specific ADAMTS13 promoter. We detected EGFP-L10a fusion protein in the liver of the transgenic mice using Western blot and further observed fusion protein-expressing cells in sinusoid cells.
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[Journal Article] ADAMTS13 gene deletion enhances plasma high-mobility group boxl elevation and neuroinflammation in brain ischemia-reperfusion injury.2012
Author(s)
Fujioka M, Nakano T, Hayakawa K, Irie K, Akitake Y, Sakamoto Y, Mishima K, Muroi C, Yonekawa Y, Banno F, Kokame K, Miyata T, Nishio K, Okuchi K, Iwasaki K, Fujiwara M, Siesjo
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Journal Title
Neurol Sci
Volume: 33
Pages: 1107-1115
DOI
Peer Reviewed
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[Journal Article] ADAMTS13 safeguards the myocardium in a mouse model of acute myocardial infarction.2012
Author(s)
Doi M, Matsui H, Takeda Y, Saito Y, Takeda M, Matsunari Y, Nishio K, Shima M, Banno F, Akiyama M, Kokame K, Miyata T, Sugimoto M
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Journal Title
Thromb Haemost
Volume: 108
Pages: 1236-1238
DOI
Peer Reviewed
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[Presentation] Protective role of ADAMTS13 for myocardium in mouse model of experimental myocardial infarction2012
Author(s)
Masaaki Doi, Hideto Matsui, Yukiji Takeda, Yoshihiko Saito, Maiko Takeda, Yasunori Matsunari, Kenji Nishio, Midori Shima, Fumiaki Banno, Masashi Akiyama, Koichi Kokame, Toshiyuki Miyata,Mitsuhiko Sugimoto
Organizer
54th American Society of Hematology, Annual Meeting and Exposition
Place of Presentation
Atlanta, USA
Year and Date
20121208-11
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[Presentation] ADAMTS13 improving the cell engraftment efficacy in mouse model of bone marrow transplantation2012
Author(s)
Hideto Matsui, Masaaki Doi, Yasunori Matsunari, Maiko Takeda, Kenji Nishio, Midori Shima, Kenji Soejima, Fumiaki Banno, Masashi Akiyama, Koichi Kokame, Toshiyuki Miyata, Mitsuhiko Sugimoto
Organizer
54th American Society of Hematology, Annual Meeting and Exposition
Place of Presentation
Atlanta, USA
Year and Date
20121208-11
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