2011 Fiscal Year Final Research Report
Integration of cell sorting with metagenomic diagnosis system
| Project/Area Number |
23659505
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Osaka University |
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Principal Investigator |
YASUNAGA Teruo 大阪大学, 微生物病研究所, 教授 (20260630)
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| Co-Investigator(Kenkyū-buntansha) |
OISHI Kazunori 大阪大学, 微生物病研究所, 特任教授 (80160414)
NAKAMURA Shota 大阪大学, 微生物病研究所, 助教 (90432434)
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| Project Period (FY) |
2011
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| Keywords | メタゲノム / 感染症診断 / セルソーティング / バイオインフォマティクス |
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| Research Abstract |
We have developed a metagenomic diagnosis system to diagnose infectious diseases by applying metagenomic analysis. This system has enabled us to directly detect pathogenic bacteria, influenza viruses and noroviruses from clinical samples. From those analyses, it has been found that contaminants such as host genome, bacteria, and/ or fungi in clinical samples reduce the efficiency and sensitivity of metagenomic detection. In order to improve the detection efficiency of the system, we developed a basic protocol of the metagenomic diagnosis system using cell-sorting technology.
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