2012 Fiscal Year Final Research Report
Study of a Rapid Test for Mycobacterium tuberculosis Complex Using an Immunochromatographic Assay
| Project/Area Number |
23659507
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Hiroshi 佐賀大学, 医学部, 教授 (40229894)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 感染症診断学 / 結核 / イムノクロマト / 迅速診断 |
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| Research Abstract |
We investigated simple and rapid test for M. tuberculosis complex by using immunochromatographic assay (ICA) to detect MPB64. Several secreted mycobacterial proteins have been reported. Among these secreted proteins, MPB64, has been identified as a M. tuberculosis complex-specific secretory protein. This kit is the newly developed ICA for rapid discrimination between the M. tuberculosis complex and the mycobacteria other than M. tuberculosis (MOTT). Primary investigation, we study rapid identification of the M. tuberculosis complex in combination with the culture systems based on liquid media. In this method, we can easily detect the M. tuberculosis complex from MOTT. Secondary, we investigated direct testing method from clinical specimens. Specimens were placed into the test well of kits, after concentrating the sediments processed and decontaminated. Direct testing method could correctly detect between the M. tuberculosis complex and MOTT, specimens contain high volume of M. tuberculosiscomplex. With a few issue, this system is useful for the rapid isolation and detection of M. tuberculosis complex.
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