2012 Fiscal Year Final Research Report
Comprehensive Analysis of Platelet Protein in High-Risk Kawasaki Disease
| Project/Area Number |
23659526
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HAMAOKA Kenji 京都府立医科大学, 医学研究科, 教授 (60189602)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Seiichiro 京都府立医科大学, 医学研究科, 助教 (40405246)
NAKAMURA Akihiro 京都府立医科大学, 医学研究科, 博士研究員 (50313854)
AKIOKA Shinji 京都府立医科大学, 医学研究科, 講師 (60598093)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 小児循環器学 / 血小板 / マイクロバーティクル / プロテオミクス / 質量分析 / 川崎病 |
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| Research Abstract |
We evaluated using blood samples of adult control in order as follows: (1)isolation of blood platelet and production of microparticle in blood, (2)deposit or sediment of protein, (3)digestion in solution or medium, (4)culture of hydrogel nano-particle, (5)nano-analysis with HPL-MS/MS, (6)search of data base and identification of protein. In result, 603 protein groups were identificated by nano-particle concentration and digestion in solution.In this study, the rapid and simple protocol for analysis of characteristics of PMP protein in blood sample.
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[Journal Article] on behalf of the RAISE study group investigators. Efficacy of immunoglobulin prednisolone for prevention of coronary artery abnormalities in severe Kawasaki disease (RAISE study): a randomized, open-label, blinded-endpoints trial2012
Author(s)
Tohru Kobayashi, Tsutomu Saji, Tetsuya Otani, Kazuo Takeuchi, Tetsuya Nakamura, Hirokazu Arakawa, Taichi Kato, Toshiro Hara, Kenji Hamaoka, Shunichi Ogawa, Masaru Miura, Yuichi Nomura, Shigeto Fuse, Fukiko Ichida, Mitsuru Seki, Ryuji Fukazawa
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Journal Title
The Lancet
Volume: 379
Pages: 1613-20
Peer Reviewed
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