2011 Fiscal Year Final Research Report

Development of stem cell therapy for epidermolysis bullosa model mice expressing humanized dermo-epidermal junctional protein

Research Project

Project/Area Number	23659539
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Dermatology
Research Institution	Hokkaido University
Principal Investigator	SHIMIZU Hiroshi 北海道大学, 大学院・医学研究科, 教授 (00146672)
Co-Investigator(Kenkyū-buntansha)	ABE Riichiro 北海道大学, 大学院・医学研究科, 准教授 (60344511) FUJITA Yasuyuki 北海道大学, 北海道大学病院, 助教 (80374437)
Project Period (FY)	2011
Keywords	皮膚炎症・再生学 / 表皮水疱症
Research Abstract	The aim of the project is to investigate the effect of stem cell therapy for congenital severe skin disorder, recessive dystrophic epidermolysis bullosa(EB), using protein-humanized EB model mice. We performed bone marrow transplantation from GFP Tg mice to EB model mice lacking murine type VII collagen(COL7) and expressing incomplete/complete human COL7. Epithelized skin areas showed GFP+bone marrow-derived keratinocytes as many as 0. 2%. Murine COL7 mRNA and COL7 protein were also expressed in some mice, but the expression levels were low. We are now performing and analyzing another bone marrow transplantation model using HMGB1, CCL27 and CCL19, which are known to enhance transdifferentiation and recruitment from bone marrow cells to skin component cells.