Challenging method allowing all skin cells to be cultured

2011 Fiscal Year Final Research Report

• Project/Area Number: 23659540
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: SAWAMUERA Daisuke 弘前大学, 大学院・医学研究科, 教授 (60196334)
• Co-Investigator(Kenkyū-buntansha): AIZU Takayuki 弘前大学, 大学院・医学研究科, 助教 (00400135) ; NAKAJIMA Koji 弘前大学, 大学院・医学研究科, 助教 (70374832)
• Project Period (FY): 2011
• Keywords: メルケル細胞癌 / 遺伝子 / トランスジェニックマウス / テトラサイクリン / タモシキフェン / 培養細胞

Research Abstract

If we can culture all cells in the skin in vitro, it must be a potential system which can develop to any studies. In this study, we tried to add tetracycline-induced promoter and LoxP sequence which is cleaved by Cre recombinase to the vector DNA and generate a transgenic mouse with MCV-Large T gene which is induced by treatment of tetracycline and is abolished by treatment of tamoxifen.

Research Products

• [Journal Article] Diffuse and focal palmoplantar keratoderma can be caused by a keratin 6c mutation (2011)
  • Author(s): Akasaka E, Nakano H, Nakano A, Toyomaki Y, Takiyoshi N, Rokunohe D, Nishikawa Y, Korekawa A, Matsuzaki Y, Mitsuhashi Y, Sawamura D
  • Journal Title: Br J Dermatol Volume: 165(6) Pages: 1290-1292
  • DOI: DOI:10.1111/j.1365-2133.2011.10552.x
• [Journal Article] DNA-based prenatal diagnosis of plectin-deficient epidermolysis bullosa simplex associated with pyloric atresia (2011)
  • Author(s): Nakamura H, Natsuga K, Nishie W, McMillan JR, Nakamura H, Sawamura D, Akiyama M, Shimizu H
  • Journal Title: Int J Dermatol Volume: 50(4) Pages: 439-442
  • DOI: DOI:10.1111/j.1365-4632.2010.04771
• [Journal Article] Immunohistochemical analysis of in vivo UVB-induced secretion of IL-1α and IL-6 in keratinocytes (2011)
  • Author(s): Narumi H, Nakano H, Matsuzaki Y, Sawamura D, Hanada K
  • Journal Title: Mol Med Report Volume: 4(4) Pages: 611-614
  • DOI: DOI:10.3892/mmr.2011.478
• [Journal Article] Vorner type palmoplantar keratoderma : novel KRT9 mutation associated with knuckle pad-like lesions and recurrent mutation causing digital mutilation (2011)
  • Author(s): Umegaki N, Nakano H, Tamai K, Mitsuhashi Y, Akasaka E, Sawamura D, Katayama I
  • Journal Title: Br J Dermatol Volume: 165(1) Pages: 199-201
  • DOI: DOI:10.1111/j.1365-2133.2011.10317.x
• [Journal Article] Dystrophic epidermolysis bullosa pruriginosa of elderly onset (2011)
  • Author(s): Hayashi M, Kawaguchi M, Hozumi Y, Nakano H, Sawamura D, Suzuki T
  • Journal Title: J Dermatol Volume: 38(2) Pages: 173-178
  • DOI: DOI:10.1111/j.1346-8138.2010.00953.x