• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Development of gene replacement system in slow growth mycobacteria to express green fluorescent protein and knock out the mce1A gene in recombinant strains of Mycobacterium leprae

Research Project

  • PDF
Project/Area Number 23659553
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionKitasato University

Principal Investigator

SATO NAOYA  北里大学, 医学部, 助教 (50276119)

Co-Investigator(Kenkyū-buntansha) FUJIMURA Takao  北里大学, 医学部, 講師 (50209087)
Project Period (FY) 2011-04-28 – 2014-03-31
Keywordsらい菌 / ハンセン病 / mce遺伝子 / 細胞内侵入因子
Outline of Final Research Achievements

Two projects have been undertaken. First, a plasmid vector for slow growth mycobacteria was made. This vector was used to generate a mutant strain of Mycobacterium leprae, which expressed green fluorescence proteins and is useful for knocking out mammalian cell entry 1A (mce1A) gene. However, It was difficult to create an efficient mutagenesis system for M. leprae, perhaps due to endogenous restriction enzymes in M. leprae. This will be a subject of future studies.
Second, antibodies raised against M. leprae Mce1A protein were made to analyze the inhibition effect of these antibodies for Mce1A protein-dependent mammalian cell invasion of M. leprae. In preliminary data, the mammalian cell invasion of a recombinant Escherichia coli, which is expressed the Mce1A protein on its surface, was inhibited by an antibody raised against a unique epitope on the Mce1A protein.

Free Research Field

医歯薬学

URL: 

Published: 2016-06-03  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi