Roles of versican in development and maintenance of hair follicles

2013 Fiscal Year Final Research Report

• Project/Area Number: 23659556
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Aichi Medical University
• Principal Investigator: WATANABE Hideto 愛知医科大学, 付置研究所, 教授 (90240514)
• Co-Investigator(Renkei-kenkyūsha): UTANI Atsushi 長崎大学, 医学部・皮膚科学講座, 教授 (10292707)
• Project Period (FY): 2011 – 2012
• Keywords: バーシカン / プロテオグリカン / 細胞外マトリックス / ノックアウトマウス / 毛包

Research Abstract

Versican (Vcan) is a large chondroitin sulfate proteoglycan of the extracellular matrix, which is transiently expressed in mesenchymal condensation areas such as cartilage primordium and dermal papilla. Here, we aimed to investigate the role of Vcan in development and maintenance of hair follicles. Though we performed intradermal injection of adenovirus expressing cre into Vcan<flox/flox> mice, we could not ablate its expression in dermal papilla cells. Currently, we are planning to generate Corin-cre; Vcan<flox/flox> mice, which lack Vcan expression in dermal papillae.

Research Products

• [Journal Article] Alterations in the chondroitin sulfate chain in human osteoarthritic cartilage of the knee (2014)
  • Author(s): Ishimaru D, Sugiura N, Akiyama H, Watanabe H, Matsumoto K
  • Journal Title: Osteoarthritis Cartilage Volume: (in press)
• [Journal Article] Chondroitinase from baculovirus Bombyx mori nucleopolyhedrovirus and chondroitin sulfate from silkworm Bombyx mori (2013)
  • Author(s): Sugiura N, Ikeda M, Shioiri T, Yoshimura M, Kobayashi M, Watanabe H
  • Journal Title: Glycobiology Volume: 23 Pages: 1520-30
  • DOI: 10.1093/glycob/cwt082.
• [Journal Article] Sulfation of the bikunin chondroitin sulfate chain determines heavy chain-hyaluronan complex formation (2013)
  • Author(s): Lord MS, Day AJ, Youssef P, Zhuo L, Watanabe H, Caterson B, Whitelock JM
  • Journal Title: J Biol Chem Volume: 288 Pages: 22930-22941
  • DOI: 10.1074/jbc.M112.404186.
• [Journal Article] Involvement of heparan sulfate 6-O-sulfation in regulation of energy metabolism and alteration of thyroid hormone levels in male mice (2013)
  • Author(s): Nagai N, Habuchi H, Sugaya N, Nakamura M, Imamura T, Watanabe H, Kimata K
  • Journal Title: Glycobiology Volume: 23 Pages: 980-992
  • DOI: 10.1093/glycob/cwt037.
• [Journal Article] Construction of a chondroitin sulfate library with defined structures and analysis of molecular interactions (2012)
  • Author(s): Sugiura N, Shioiri T, Chiba M, Sato T, Narimatsu H, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 287 Pages: 43390-43400
  • DOI: 10.1074/jbc.M112.412676.
• [Journal Article] Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development (2012)
  • Author(s): Ogawa H, Hatano S, Sugiura N, Nagai N, Sato T, Shimizu K, Narimatsu H, Kimata K, Watanabe H
  • Journal Title: PLoS ONE Volume: 7 Pages: e43806
  • DOI: 10.1371/journal.pone.0043806.
• [Journal Article] Versican/PG-M is essential for ventricular septal formation subsequent to atrioventricular cushion development (2012)
  • Author(s): Hatano S, Kimata K, Hiraiwa N, Kusakabe M, Isogai Z, Adachi E, Shinomura T, Watanabe H
  • Journal Title: Glycobiology Volume: 22 Pages: 1268-1277
  • DOI: 10.1093/glycob/cws095.
• [Journal Article] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth (2012)
  • Author(s): Kono A, Oguri A, Yokoo K, Watanabe H
  • Journal Title: J Plast Surg Hand Surg Volume: 46 Pages: 308-312
  • DOI: 10.3109/2000656X.2012.696197
• [Journal Article] Keratan Sulfate Restricts Neural Plasticity after Spinal Cord Injury (2011)
  • Author(s): Imagama S, Sakamoto K, Tauchi R, Shinjo R, Ohgomori T, Ito Z, Zhang H, Nishida Y, Asami N, Takeshita S, Sugiura N, Watanabe H, Yamashita T, Ishiguro N, Matsuyama Y, Kadomatsu K
  • Journal Title: J Neurosci Volume: 31 Pages: 17091-17102
  • DOI: 10.1523/JNEUROSCI.5120-10.20
• [Journal Article] Cell surface heparan sulfate chains regulate local reception of FGF signaling in the mouse embryo (2011)
  • Author(s): Shimokawa K, Kimura-Yoshida C, Nagai N, Mukai K, Matsubara K, Watanabe H, Matsuda Y, Mochida K, Matsuo I
  • Journal Title: Dev Cell Volume: 21 Pages: 257-272
  • DOI: 10.1016/j.devcel.2011.06.027.
• [Journal Article] Baculovirus envelope protein ODV-66 is a novel chondroitinase with distinct substrate specificity (2011)
  • Author(s): Sugiura N, Setoyama Y, Chiba M, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 286 Pages: 29026-29034
  • DOI: 10.1074/jbc.M111.251157.
• [Presentation] Chondroitin Sulfate synthase-2/chondroitin polymerizing factor : characterization and impact on chondroitin sulfate biosynthesis (2012)
  • Author(s): Hideto Watanabe, Hiroyasu Ogawa, Masafumi Shionyu, Nobui Sugiura, Sonoko Hatano, Naoko Nagai, Takashi Sato, Hisashi Narimatsu, Katsuji Shimizu, Koji Kimata
  • Organizer: 2012 Joint Meeting of the Society for Glycobiology & American Society for Matrix Biology
  • Place of Presentation: San Diego
  • Year and Date: 20121111-14
• [Presentation] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth (2012)
  • Author(s): Ayuko Kono, Akiko Oguri, Kazuhisa Yokoo, Hideto Watanabe
  • Organizer: 23^<rd> FECTS and ISMB Joint Meeting
  • Place of Presentation: Katowice, Poland
  • Year and Date: 20120825-29
• [Presentation] Chondroitin sulfate synthase-2/chondroitin polymerizing factor : characterization and impact on chondroitin sulfate biosynthesis (2011)
  • Author(s): Hiroyasu Ogawa, Masashi Shionyu, Nobuo Sugiura, Sonoko Hatano, Naoko Nagai, Takashi Sato, Masanori Gotoh, Hisashi Narimatsu, Katsuji Shimizu, Koji Kimata, Hideto Watanabe
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: Sydney
  • Year and Date: 20111016-23
• [Remarks] 愛知医科大学分子医科学研究所
  • URL: http://www.aichi-med-u.ac.jp/su10/su1009/index.html