2012 Fiscal Year Final Research Report
Analysis of iPS cells from patients with 22q11.2 deletion and schizophrenia
| Project/Area Number |
23659570
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Psychiatric science
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
YOSHIKAWA Takeo 独立行政法人理化学研究所, 分子精神科学研究チーム, チームリーダー (30249958)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MAEKAWA Motoko 独立行政法人理化学研究所, 分子精神科学研究チーム, 研究員 (50435731)
TOYOSHIMA Manabu 独立行政法人理化学研究所, 分子精神科学研究チーム, 基礎科学特別研究員 (90582750)
|
|---|
| Research Collaborator |
岡野 栄之 慶応大学, 医学部生理学教室
赤松 和土 慶応大学, 医学部生理学教室
岡田 洋平 慶応大学, 医学部生理学教室
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 22q11.2欠失 / 統合失調症 / iPS cell / neurosphere / DGCR8 / let-7 family |
|---|
| Research Abstract |
We established iPS cells from two patients with 22q11.2 deletion and schizophrenia. Control iPS cells were from two normal subjects. We differentiated them into neurospheres (NS). Using these NS, we performed cDNA microarray analysis and found that DGCR8 gene, which is responsible for miRNA processing, is down-regulated in the NS from patients. Then we did miRNA chip analysis and revealed that the expressions of miRNAs relevant to differentiation from NS to neurons are dysregulated.
|
