Depression mechanism of survival signals induced by radiation

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659594
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Nara Medical University
• Principal Investigator: ONISHI Takeo 奈良県立医科大学, 医学部, 特任教授 (60094554)
• Project Period (FY): 2011 – 2012
• Keywords: 放射線治療生物学 / 重粒子線 / 粒子線治療 / 細胞死シグナル / 生存シグナル / Akt

Research Abstract

Human gingival cancer cells (Ca9-22 cells) harboring mutated p53 (mp53) gene were irradiated with 2 Gy of X-rays or Fe-ion beams. Akt-related protein levels decreased when cells were irradiated with high LET radiation. In addition, high LET radiation increased G2/M phase arrests and suppressed the progression of the cell cycle much more efficiently when compared to low LET radiation. These results suggest that high LET radiation enhances apoptosis and cell cycle arrest by suppressing Akt-related signals,even in the cancer cells bearing mp53 gene.

Research Products

• [Journal Article] Depression of p53-independent Akt-survival signals in human oral cancer cells bearing mutatedp53 gene after exposure to high-LET radiation (2012)
  • Author(s): Nakagawa, Y., A. Takahashi, A. Kajihara, N. Yamakawa, Y. Imai, I. Ota, N. Okamoto, E. Mori, T. Noda, Y. Furusawa, T. Kirita& T. Ohnishi
  • Journal Title: Biochem Biophys Res Commun Volume: 423(4) Pages: 654-660
  • Peer Reviewed
• [Journal Article] Expression of p53-regulated proteins in human cultured lymphoblastoid TSCE5 and WTK1 cell lines during spaceflight (2012)
  • Author(s): Takahashi, A., H. Suzuki, K. Omori, M. Seki, T. Hashizume, T. Shimazu, N. Ishioka & T. Ohnishi
  • Journal Title: J. Radiat. Res Volume: 53(2) Pages: 168-175
  • Peer Reviewed
• [Journal Article] Preliminary results of space experiment: Implications for the effects of space radiation and microgravity on survival and mutation induction in human cells (2012)
  • Author(s): Yatagai, F., M. Honma, A. Ukai, K. Omori, H. Suzuki, T. Shimazu, A. Takahashi, T. Ohnishi, N. Dohmae & N. Ishiok
  • Journal Title: Adv. Space Res Volume: 49(3) Pages: 479-486
  • Peer Reviewed
• [Journal Article] 福島第一原子力発電所事故から学び、提案する (2012)
  • Author(s): 大西武雄
  • Journal Title: 食品・食品添加物研究誌FFI ジャーナル Volume: 217(2) Pages: 189-197
• [Journal Article] Therapeutic strategies for head and neck cancer based on p53 status (2012)
  • Author(s): Ota, I., N. Okamoto, K. Yane, A. Takahashi, T. Masui, H. Hosoi & T. Ohnishi
  • Journal Title: Exp. Therap. Med Volume: 3(4) Pages: 585-591
  • Peer Reviewed
• [Journal Article] siRNA targeted for NBS1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells (2011)
  • Author(s): Okamoto, N., A. Takahashi, I. Ota, K. Ohnishi, E. Mori, N. Kondo, X. Su, K. Yane, H. Hosoi & T. Ohnishi
  • Journal Title: Int. J. Hyperthermia Volume: 27(3) Pages: 297-304
  • Peer Reviewed
• [Journal Article] Frozen human cells can record radiation damage accumulated during space flight; mutation induction and radioadaptation (2011)
  • Author(s): Yatagai, F., M. Honma, A. Takahashi, K. Omori, H. Suzuki, T. Shimazu, M. Seki, T. Hashizume, A. Ukai, K. Sugasawa, T. Abe, N. Dohmae, S. Enomoto, T. Ohnishi, A. Gordon & N. Ishioka
  • Journal Title: Radiat. Environ. Biophys Volume: 50(1) Pages: 125-134
  • Peer Reviewed
• [Journal Article] Nitric Oxide Radical-induced Radioadaptation and Radiosensitization Are G2/M Phase-dependent (2011)
  • Author(s): Su, X., A. Takahashi, N. Kondo, Y. Nakagawa, T. Iwasaki, G. Guo & T. Ohnishi
  • Journal Title: J. Radiat. Res Volume: 52(5) Pages: 609-615
  • Peer Reviewed
• [Journal Article] FANCD1/BRCA2 Plays Predominant Role in the Repair of DNA Damage Induced by ACNU or TMZ (2011)
  • Author(s): Kondo, N., A. Takahashi, E. Mori, T. Noda, M. Z. Zdzienicka, L. H. Thompson, T. Helleday, M. Suzuki, Y. Kinashi, S. Masunaga, K. Ono, M. Hasegawa & T. Ohnishi
  • Journal Title: PLoS One Volume: 6(5) Pages: 19659
  • Peer Reviewed
• [Journal Article] Achievement of the first life science experiments in the Japanese Experimental Module "Kibo" of the ISS (2011)
  • Author(s): Takahashi, A. & T. Ohnishi
  • Journal Title: Int. Sym. Space Tec. Sci Pages: 18-20
• [Journal Article] Long-term stay against space radiation for human health (2011)
  • Author(s): Ohnishi T. & A.Takahashi
  • Journal Title: Int. Sym. Space Tec. Sci Pages: 14-16
• [Journal Article] Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state (2011)
  • Author(s): Takahashi, A., H. Suzuki, K. Omori, M. Seki, T. Hashizume, T. Shimazu, N. Ishioka & T. Ohnishi
  • Journal Title: Adv. Space Res Volume: 47(6) Pages: 1062-1070
  • Peer Reviewed
• [Journal Article] Repair pathways independent of the Fanconi anemia nuclear core complex play a predominant role in mitigating formaldehyde-induced DNA damage (2011)
  • Author(s): Noda, T., A. Takahashi, N. Kondo, E. Mori, N. Okamoto, Y. Nakagawa, K. Ohnishi, M. Z. Zdzienicka, L. H. Thompson, T. Helleday, H. Asada & T. Ohnishi
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 404(1) Pages: 206-210
  • Peer Reviewed
• [Journal Article] 宙に挑む生命の力 (2011)
  • Author(s): 大西武雄
  • Journal Title: 環境と健康 Volume: 24(2) Pages: 151-157
• [Journal Article] 地震と津波による福島第一原子力発電所で大事故発生:大気・土壌・食料・水道水・海水の放射能による汚染 (2011)
  • Author(s): 大西武雄
  • Journal Title: 食品・食品添加物研究誌 FFI ジャーナル Volume: 216(3) Pages: 250-257
• [Journal Article] 放射線生物学の歴史に残る第一原子力発電所の大事故 (2011)
  • Author(s): 大西武雄
  • Journal Title: 放射線生物研究 Volume: 45(2) Pages: 97-106
• [Presentation] new aspect of space life science research in ISS 、China-Japan-Korea Workshop on Microgravity Sciences Asian Microgravity Pre-Symposium (2012)
  • Author(s): Ohnishi T
  • Organizer: China-Japan-Korea Workshop on Microgravity Sciences Asian Microgravity Pre-Symposium
  • Place of Presentation: Ronghu Lake Hotel, Guilin, Guangxi, China
  • Year and Date: 2012-11-01
• [Presentation] A new aspect of space life science research in ISS (2012)
  • Author(s): Indore,Public Lecture, Indore, India
  • Organizer: China-Japan-Korea Workshop on Microgravity Sciences Asian Microgravity Pre-Symposium
  • Place of Presentation: Ronghu Lake Hotel, Guilin
  • Year and Date: 2012-11-01
• [Presentation] Stress-induced, NO-mediated bystander responses forprotecting cells themselves (2012)
  • Author(s): Matsumoto, A. Takahashi & T. Ohnishi
  • Organizer: 11th Int. Congr. Hyperthermic Oncol. & 29th Jpn. Congr. Thermal Med
  • Place of Presentation: Hyatt Regency Kyoto, Kyoto, Japan
  • Year and Date: 2012-08-29
• [Presentation] DNA-PK activity is involved in the fast repair of DNA double strand breaks during thermal radiosensitization (2012)
  • Author(s): Ihara, M., Y. Okumura & T. Ohnishi
  • Organizer: 11th Int. Congr. Hyperthermic Oncol. & 29th Jpn. Congr. Thermal Med
  • Place of Presentation: Hyatt Regency Kyoto, Kyoto, Japan
  • Year and Date: 2012-08-29
• [Presentation] Enhancement of heat sensitivity by depression of DSB repair (2012)
  • Author(s): Kajihara, A., N. Okamoto, Y. Nakagawa, A. Takahashi, T. Kirita & T. Ohnishi
  • Organizer: 11th Int. Congr. Hyperthermic Oncol. & 29th Jpn. Congr. Thermal Med
  • Place of Presentation: Hyatt Regency Kyoto, Kyoto, Japan
  • Year and Date: 2012-08-29
• [Presentation] ATM is the predominant kinase involved in the phosphorylation of histone H2AX after heating (2012)
  • Author(s): Takahashi, A., E. Mori, D.J. Chen & T. Ohnishi
  • Organizer: 11th Int. Congr. Hyperthermic Oncol. & 29th Jpn. Congr. Thermal Med
  • Place of Presentation: Hyatt Regency Kyoto, Kyoto, Japan
  • Year and Date: 2012-08-29
• [Presentation] A priming heat treatment can induce the development of heat- and radio-resistance via HSPs regardless of p53-gene status (2012)
  • Author(s): Takahashi, A. & T. Ohnishi
  • Organizer: 11th Int. Congr. Hyperthermic Oncol. & 29th Jpn. Congr. Thermal Med
  • Place of Presentation: Hyatt Regency Kyoto, Kyoto, Japan
  • Year and Date: 2012-08-29
• [Presentation] Recent condition of Fukushima-Daiichi nuclear plant accident in Japan (2012)
  • Author(s): Ohnishi, T
  • Organizer: 39th COSPAR 、Narayana Murthy Centre of Excellence (Infosys Training Centre)
  • Place of Presentation: Mysore, India
  • Year and Date: 2012-07-21
• [Presentation] Fukushima-Daiichi nuclear power plant accident and the recent condition (2012)
  • Author(s): Ohnishi T
  • Organizer: 7th Biennial Conf. of Indian Associat. Hyperthemia Oncol. and Med
  • Place of Presentation: Indore,Public Lecture, Indore, India
  • Year and Date: 2012-02-04
• [Presentation] Molecular mechanism of heat sensitivity from a new aspect of DNA repair for DSBs (2012)
  • Author(s): Ohnishi, T
  • Organizer: 7th Biennial Conf. of Indian Associat. Hyperthemia Oncol. and Med.
  • Place of Presentation: Indore,Public Lecture, Indore, India
  • Year and Date: 2012-02-04
• [Presentation] FANCD1/BRCA2 plays predominant role in the repair of DNA damage induced by ACNU or TMZ (2011)
  • Author(s): Kondo, N., A. E. Takahashi, T. Mori, M. Noda, Z. Zdzienicka, L. H. Thompson, T. Helleday, M. Suzuki, Y. Kinashi, S. Masunaga, K. Ono, M. Hasegawa & T.Ohnishi
  • Organizer: 14th Int. Conr. Radiat. Res
  • Place of Presentation: The Palace of Culture and Science, Warsaw, Poland
  • Year and Date: 2011-08-30
• [Presentation] Space radiation effects on human cultured cells in an International Space Station at Kibomodule (2011)
  • Author(s): Takahashi, A., H. Suzuki, O. Katsunori, M. Seki, T. Hashizume, T. Shimazu, N. Ishioka & T. Ohnishi
  • Organizer: 14th Int. Conr. Radiat. Res
  • Place of Presentation: The Palace of Culture and Science, Warsaw, Poland
  • Year and Date: 2011-08-29
• [Presentation] Space radiation effects on human cultured cells in an International Space Station at Kibo module (2011)
  • Author(s): Katsunori, M. Seki, T. Hashizume, T.Shimazu, N. Ishioka & T. Ohnishi
  • Organizer: 14th Int. Conr. Radiat. Res
  • Place of Presentation: The Palace of Culture and Science, Warsaw, Poland
  • Year and Date: 2011-08-29
• [Presentation] Desaster of Fukushima-Daiichi nuclear power plant (Japan) by earthquake, tsunami and RI-pollution (2011)
  • Author(s): Ohnishi T
  • Organizer: Desaster of Fukushima-Daiichi nuclear power plant (Japan) by earthquake, tsunami and RI-pollution
  • Place of Presentation: The Palace of Culture and Science, Warsaw, Poland
  • Year and Date: 2011-08-28
• [Presentation] Achievement of the first life science experiment "Rad Gene" in the Japanese (2011)
  • Author(s): Takahashi, A. & T. Ohnishi
  • Organizer: Experimental Module "Kibo" of the ISS、28th Int. Symp. Space Tech. and Sci. Naha
  • Place of Presentation: Okinawa Convention Center ,Okinawa, Japan
  • Year and Date: 2011-06-10
• [Presentation] Earthquake and tsunami hit Fukushima-Daiichi nuclear power plants in Japan; RI-pollution of air/food/water/seawater (2011)
  • Author(s): Ohnishi T
  • Organizer: Int. Natl. Symp. Radiat. Res. Med. Phys
  • Place of Presentation: Shanghai Cancer Center, Fudan University, Shanghai, China
  • Year and Date: 2011-05-31
• [Book] 化学物質・放射線.理科年表 (2012)
  • Author(s): 大西武雄
  • Total Pages: 1037-1038
  • Publisher: 丸善
• [Book] 人間はたくましい生きものなんだ.モーニング編集部 X 門倉紫麻編集, We are 宇宙兄弟, 宇宙を舞台に活躍する人たち (2011)
  • Author(s): 大西武雄
  • Total Pages: 68-85
  • Publisher: 講談社