2013 Fiscal Year Final Research Report
Diagnostic bioimaging for acute cellular rejection using antibody probes in mouse liver transplantation
| Project/Area Number |
23659607
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OZAKI Michitaka 北海道大学, 保健科学研究院, 教授 (80256510)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 肝移植 / 拒絶反応 |
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| Research Abstract |
We conducted this study to develop a new diagnostic method for allograft rejection in liver transplantation where infiltrating T-cells were quantified using antibody probes. We succeeded in inducing an acute cellular rejection in a mouse model of allogeneic liver transplantation. Immunohistologically, we confirmed that CD3 was strongly expressed on the infiltrating T-cells. However, a biological imaging after systemic injection of anti CD3 antibody probes could not detect the infiltrating T-cells. To deliver antibody probes to the infiltrating T-cells directly, we developed an extracorporeal perfusion machine for mouse liver and administered antibody probes to liver graft via the perfusion fluid. Even in this method, anti CD3 antibody probes could not detect the infiltrating T-cells.
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