2012 Fiscal Year Final Research Report
The production of the pigs with an artificial gene (a functional domain-polymeriged nucleotide based on optimal codonusage)
| Project/Area Number |
23659612
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MIYAGAWA Shuji 大阪大学, 大学院・医学系研究科, 准教授 (90273648)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 異種移植 / トランスジェニックブタ / ハイブリッド遺伝子 / 顕微授精 |
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| Research Abstract |
T o establish transgenic pigs with multiple human genes, the hybrid gene containing the function domains of each molecule were produced and the pigs with the gene was then tried to produce by intracytoplasmic sperm injection(ICSI) methods. At first, the transgenic mouse with the hybrid genes were successfully produced and analyzed. Concerning the pig project, the condition of the oocyte with each gene was studied under the microscope. The condition of the segmentation was no problem, however the pigs with genes were not born in this period.
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[Journal Article] A cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase from porcine endothelial cells2012
Author(s)
Ikeda K, Yamamoto A, Nanjo A, Inuinaka C, Takama Y, Ueno T, Fukuzawa M, Nakano K, Matsunari H, Nagashima H, Miyagawa S.
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Journal Title
Transplant Proc.
Volume: 44
Pages: 1136-8
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[Presentation] In comparison with APIs, NICCs are very rich in a2,6Neu5NAc, and reduced Fucose/Core Fucose & high-mannose form, and Lactosamine & Core 1 forms are upregulated, instead, in 5 day cultures.2011
Author(s)
Hiroaki Kashiwada, Akiko Nanjyo, Shino Nakatsu, Yuichi Takama, Teru Okitsu, Hiroshi Nagashima, Masahiro Fukuzawa, Shuji Miyagawa.
Organizer
The 11th Congress of International Congress for Xenotransplantation (Joint Conference with CST)
Place of Presentation
Miami, USA
Year and Date
20111023-26
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[Presentation] A cloning of cytidine monophospho-N-acetylneuraminic acid (CMP-NeuAc) hydroxylase from porcine endothelial cells
Author(s)
Kosuke Ikeda, Akiko Nanjyo, Hiroaki Kashiwada, Yuichi Takama, Takehisa Ueno, Masahiro Fukuzawa, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Nagashima, Shuji Miyagawa
Organizer
12th Congress of the Asian Society of Transplantation
Place of Presentation
Seoul, Korea.
Year and Date
00000925-28
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[Presentation] Trial of knockdown for the H-D antigen of pig cells
Author(s)
Akiko Nanjyo, Aki Yamamoto, Hiroaki Kashiwada, Kosuke Ikeda, Yuichi Takama, Takehisa Ueno, Masahiro Fukuzawa, Akihiro Kondo, Hiroshi Nagashima, Shuji Miyagawa
Organizer
12th Congress of the Asian Society of Transplantation
Place of Presentation
Seoul, Korea.
Year and Date
00000925-28
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[Presentation] A study of the glycoantigens of neonatal porcine islet-like cell clusters using a lectin microarray
Author(s)
Hiroaki Kashiwada, Akiko Nanjyo, Shino Nakatsu, Shunsaku Takeishi, Yuichi Takama, Akihiro Kondo, Teru Okitsu, Hiroshi Nagashima, Masahiro Fukuzawa, Shuji Miyagawa.
Organizer
12th Congress of the Asian Society of Transplantation
Place of Presentation
Seoul, Korea.
Year and Date
00000925-28
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[Presentation] Adult pig islets, rich in the high-mannose form compared with humans, were slightly up-regulated, and alpha-linked GalNAc was reduced byGal-knockout
Author(s)
Wang D, Maeda A, Ueno T, Takama Y, Fukuzawa M, Matsumoto S, Okitsu T, Goto M, Kondo K, Nagashima H, Miyagawa S.
Organizer
The 24thInternational Congress of the Transplantation Sociaty
Place of Presentation
Berlin
Year and Date
00000715-19
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