Novel Innovative Safety Reprograming Technology by MicroRNAs

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659617
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: ISHII Hideshi 大阪大学, 医学系研究科, 寄附講座教授 (10280736)
• Co-Investigator(Kenkyū-buntansha): MORI Masaki 大阪大学, 大学院・医学系研究科, 教授 (70190999)
• Project Period (FY): 2011 – 2012
• Keywords: 再生医学 / 外科学 / ES 細胞

Research Abstract

For innovative cancer therapy, we studied the novel methods to induce microRNA-based induced pluripotent stem cells (miPS) by introduction of synthetic oligo microRNA. This novel technology would be able to apply the broad range of gastrointestinal diseases such as human cancer.

Research Products

• [Journal Article] SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers. (2013)
  • Author(s): Suzuki, Y., Haraguchi, N., Takahashi, H., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Mizushima, T., Ishii, H., Doki, Y., Mori, M., Yamamoto, H
  • Journal Title: Int. J. Oncol. Volume: 42(1) Pages: 161-167
• [Journal Article] Depletion of Jarid1b induces cellular senescence in human colorectal cancer. (2013)
  • Author(s): Ohta, K., Haraguchi, N., Kano, Y., Kagawa, Y., Konno, M., Nishikawa, S., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Nishimura, T., Noguchi, Y., Ozaki, M., Kudo, T., Sakai, D., Satoh, T., Fukami, M
  • Journal Title: Intern. J. Oncol. Volume: 42(4) Pages: 1212-1218
• [Journal Article] Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance. (2012)
  • Author(s): Hashiguchi, Y., Nishida, N., Mimori, K., Sudo, T., Tanaka, F., Shibata, K., Ishii, H., Mochizuki, H., Hase, K., Doki, Y., Mori, M.
  • Journal Title: Int. J. Oncol Volume: 40(5) Pages: 1477-1482
• [Journal Article] Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma. (2012)
  • Author(s): Yokobori, T., Mimori, K., Iwatsuki, M., Ishii, H., Tanaka, F., Sato, T., Toh, H., Sudo, T., Iwaya, T., Tanaka, Y., Onoyama, I., Kuwano, H., Nakayama, K.I., Mori, M.
  • Journal Title: Int. J. Oncol Volume: 41(1) Pages: 253-259
• [Journal Article] Nagano, H., Doki, Y., Mori, M. Genotoxic therapy stimulates error-prone DNA repair in dormant hepatocellular cancer stem cells. (2012)
  • Author(s): Nishikawa, S., Ishii, H., Haraguchi, N., Kano, Y., Fukusumi, T., Ohta, K., Ozaki, M., Sakai, D., Satoh, T.,Nagano, H., Doki, Y., Mori, M.
  • Journal Title: Exp. Ther. Med. Volume: 3(6) Pages: 959-962
• [Journal Article] Absence of CD71 transferrin receptor characterizes human gastric adenosquamous carcinoma stem cells (2012)
  • Author(s): Ohkuma, M., Haraguchi, N., Ishii, H., Mimori, K., Tanaka, F., Kim, H.M., Shimomura, M., Hirose, H., Yanaga, K., Mori, M.
  • Journal Title: Ann. Surg. Oncol. Volume: 19(4) Pages: 1357-1364
• [Journal Article] Increased CD13 expression reduces reactive oxygen species, promoting survival of liver cancer stem cells via an epithelial-mesenchymal transition-like phenomenon. (2012)
  • Author(s): Kim, H.M., Haraguchi, N., Ishii, H., Ohkuma, M., Okano, M., Mimori, K., Eguchi, H., Yamamoto, H., Nagano, H., Sekimoto, M., Doki, Y., Mori, M.
  • Journal Title: Ann. Surg. Oncol. Volume: 19 Pages: S539-S548
• [Journal Article] Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic microRNA clusters. (2012)
  • Author(s): Nishida, N., Nagahara, M., Sato, T., Mimori, K., Sudo, T., Tanaka, F., Shibata, K., Ishii, H., Sugihara, K., Doki, Y., Mori, M.
  • Journal Title: Clin. Cancer Res. Volume: 18(11) Pages: 3054-3070
• [Journal Article] Transcriptome study of dormant gastrointestinal cancer stem cells. (2012)
  • Author(s): Nishikawa, S., Dewi, D.L., Ishii, H., Konno, M., Haraguchi, N., Kano, Y., Fukusumi, T., Ohta, K., Noguchi, Y., Ozaki, M., Sakai, D., Satoh, T., Doki, Y., Mori, M.
  • Journal Title: Int. J. Oncol. Volume: 41(3) Pages: 979-984
• [Journal Article] Dicer 1, ribonuclease type III modulates a reprogramming effect in colorectal cancer cells. (2012)
  • Author(s): Dewi, D.L., Ishii, H., Haraguchi, N., Nishikawa, S., Kano, Y., Fukusumi, T., Ozaki, M., Saito, T., Sakai, D., Satoh, T., Doki, Y., Mori, M.
  • Journal Title: Int J Mol Med. Volume: 29(6) Pages: 1060-1064
• [Journal Article] Hypoxia and TP53 deficiency for induced pluripotent stem cell-like properties in gastrointestinal cancer. (2012)
  • Author(s): Hoshino, H., Nagano, H., Haraguchi, N., Nishikawa, S., Tomokuni, A., Kano, Y., Fukusumi, T., Saito, T., Osaki, M., Sakai, D., Satoh, T., Eguchi, H., Sekimoto, M., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Intern. J. Oncol. Volume: 40(5) Pages: 1423-1230
• [Presentation] Leucine-rich repeat-containing G-protein-coupled receptor5(Lgr5)のヒト腸管における発現解析 (2012)
  • Author(s): 浜部敦史 他13名
  • Organizer: 第23回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-15
• [Presentation] 胃癌幹細胞の同定とその生物学的解析 (2012)
  • Author(s): 西川晋平 他14名
  • Organizer: 第23回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-15
• [Presentation] Cancer stem cell research for innovative cell-modifying technologies (2012)
  • Author(s): 石井秀始
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-19
• [Presentation] 先端医療:がん幹細胞理論に基づく新規治療開発 (2012)
  • Author(s): 石井秀始
  • Organizer: 第10回日本臨床腫瘍学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-19
• [Presentation] Induced Pluripotent Stem Cell Reprogramming for Innovative Cell-Modifying Technology in Cancer (2012)
  • Author(s): 石井秀始
  • Organizer: 第33回日本炎症・再生医学学会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2012-07-05