2012 Fiscal Year Final Research Report
In vivo imaging of liver organogenesis from human liver bud
| Project/Area Number |
23659620
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
KOIKE Naoto 横浜市立大学, 医学研究科, 客員研究員 (50301081)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Yasuharu 横浜市立大学, 医学部, 助教 (60375235)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEBE Takanori 横浜市立大学, 医学部, 助手 (20612625)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 外科総論 / 肝組織再構築 / 微小血管網 / イメージング |
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| Research Abstract |
In this study, we aimed to establish a visualization system to monitor liver organogenesis from liver bud in vivo. To this end, we developed a liver bud generation method according to the three-dimensional co-culture of human liver stem cells, human umbilical vein endothelial cells (HUVEC) and human mesenchymal stem cells (hMSC). Next, we established a 4D imaging system using ultra-high-speed confocal microscope system. Then we transplanted the human liver bud into the cranial window and observed visualized and reconstituted human liver tissue with human vascular network. We have established an imaging technology to visualize and analyze the formation of human solid organs.
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[Journal Article] Imaging Studies of Intraductal Tubulopapillary Neoplasms of the Pancreas: 2-Tone Duct Sign and Cork-of-Wine-Bottle Sign as Indicators of Intraductal Tumor Growth2012
Author(s)
Motosugi U, Yamaguchi H, Furukawa T, Ichikawa T, Hatori T, Fujita I, Yamamoto M, Motoi F, Kanno A, Watanabe T, Koike N, Koyama I, Kobayashi J, Shimizu M.
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Journal Title
J Comput Assist Tomogr
Volume: 36
Pages: 710-717
Peer Reviewed
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[Journal Article] Vascularized and functional human liver from an iPSC-derived organ bud transplant
Author(s)
Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zhang RR, Ueno Y, Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguchi H.
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Journal Title
Peer Reviewed
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[Presentation] Quantitative targeted absolute proteomics of transporters in the pancreatic cancer treated with neoadjuvant chemoradiation therapy2012
Author(s)
Ueno Y, Sun L, Ouchi R, Nakata S, Zheng YW, Terasaki T, Kurata M, Yamamoto N, Morinaga S, Miyagi Y, Yokose T, Endo I, Taniguchi H.
Organizer
第71回日本癌学会学術総会
Place of Presentation
札幌市教育文化会館(北海道)
Year and Date
20120919-21
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[Presentation] Creation of vascularized human organ from induced pluripotent stem cells2012
Author(s)
Takebe T, Sekine K, Enomura M, Suzuki Y, Koike H, Zhang R, Koike N, Ueno Y, Zheng Y, Taniguchi H
Organizer
nternational Society for Stem Cell Research 10th annual meeting
Place of Presentation
Yokohama,Japan, Pacifico Yokohama(KANAGAWA)
Year and Date
20120613-16
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[Presentation] Self-renewal versus differentiation as well as the liver repopulation capability of human hepatic stem cells2012
Author(s)
Zheng Y, Li B, Zhang R, Kimura M, Tsuchida T, Takebe T, Ueno Y, Sekine K, Taniguchi H
Organizer
International Society for Stem Cell Research 10th annual meeting
Place of Presentation
Yokohama,Japan, Pacifico Yokohama(KANAGAWA)
Year and Date
20120613-16
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[Presentation] Construction of chimeric mice with human immatured hepatocytes2012
Author(s)
Zhang R, Zheng Y, Tsuchida T, Takebe T, Kimura M, Li B, Takiguchi K, Sekine K, Ueno Y, Taniguchi H
Organizer
International Society for Stem Cell Research 10th annual meeting
Place of Presentation
Yokohama,Japan, Pacifico Yokohama(KANAGAWA)
Year and Date
20120613-16
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