2013 Fiscal Year Final Research Report
In vivo analysis of EMT by a newly designed optic probes with dual secretory
| Project/Area Number |
23659630
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
KATAOKA akihiko 北海道大学, 医学研究科, 非常勤講師 (90399832)
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| Co-Investigator(Kenkyū-buntansha) |
HAGA Sanae 北海道大学, 大学院保健科学研究院, 博士研究員 (60706505)
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| Co-Investigator(Renkei-kenkyūsha) |
OZAKI Michitaka 北海道大学, 大学院保健科学研究院, 教授 (80256510)
OHMIYA Yoshihiro 産業技術総合研究所, バイオメディカル研究部門, 研究部門長 (20223951)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 光イメージング / 生体イメージング / 腫瘍モニタリング / マウス / 分泌型ルシフェラーゼ |
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| Research Abstract |
We developed a mouse model for in vivo optic imaging by implanting subcutaneously tumor cells. Tumor cells express constitutively optic probes (secretory C-Luc probe and conventional F-Luc probe). We monitored tumor cell growth, and signals from optic probes (F-Luc) by imaging and by blood (C-Luc). We could image tumors by optic imaging parallel to tumor size and also monitor tumor expansion non-invasively by an aliquot of blood (1-2ul).
In the present study, we could check tumor expression/depression by the newly developed optic probes by in vivo imaging and by measuring blood samples. These probes will contribute to a future diagnosis/therapy as a new monitoring tool or to develop a new drug.
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[Journal Article] NK026680 inhibits T-cell function in an IL-2-dependent manner and prolongs cardiac allograft survival in rats2012
Author(s)
Shibasaki S, Yamashita K, Goto R, Oura T, Wakayama K, Hirokata G, Shibata T, Igarashi R, Haga S, Ozaki M, Todo S
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Journal Title
Transpl Immunol
Volume: 26
Pages: 42-49
Peer Reviewed
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[Journal Article] Anoikis induction and inhibition of peritoneal metastasis of pancreatic cancer cells by a novel nuclear factor-kappa B inhibitor, (-)-DHMEQ
Author(s)
Masanori Sato, Kazuaki Nakanishi, Sanae Haga, Masato Fujiyoshi, Motoi Baba, Kazuhiro Mino, Yimin, Haruki Niwa, Hideki Yokoo, Kazuo Umezawa, Yoshihiro Ohmiya, Toshiya Kamiyama, Satoru Todo, Akinobu Taketomi, Michitaka Ozaki, Akihiko Kataoka
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Journal Title
Oncology Research
Volume: (accepted)
Peer Reviewed
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