2012 Fiscal Year Final Research Report
Analysis of regulatory mechanism for liver regeneration by various types of cell death(autophagy and apoptosis)
| Project/Area Number |
23659631
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
OZAKI Michitaka 北海道大学, 大学院・保健科学研究院, 教授 (80256510)
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| Project Period (FY) |
2011 – 2012
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| Keywords | オートファジー / アポトーシス / p62/SQSTM1 / Nrf-2 |
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| Research Abstract |
In order to examine the regulatory role of autophagy and p62/SQSTM1 in fatty liver regeneration, we studied steatotic cell death (autophagy and apoptosis) in liver/hepatocytes.(1) Establishment of evaluation method for autophagy in cell/liver tissue. We tried to quantitatively evaluate autophagy of cells and liver tissue by expressions of LC3 and p62/SQSTM1, and electron micrograph.(2) We studied the regulatory mechanism of p62/SQSTM1 expression and its relation to apoptosis/autophagy and oxidative stress. p62/SQSTM1 expression was regulated by autophagy specifically, not by apoptosis, but also regulated positively by PI3-K/PDK/Akt-dependent signals. p62/SQSTM1 was also involved in suppression of cellular oxidative stress and injury. In mouse liver, p62/SQSTM1 increased cellular anti-oxidative molecules such as SOD, Ref-1 and Catalase through Nrf-2 and suppressed oxidative stress.
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[Journal Article] Inhibition of macrophage activation and suppression of graft rejection by DTCM-glutarimide, a novel piperidine derived from the antibiotic 9-methylstreptimidone.2011
Author(s)
Masatoshi Takeiri, Miyuki Tachibana, Ayumi Kaneda, Ayumi Ito, Yuichi Ishikawa, Shigeru Nishiyama, Ryoichi Goto, Kenichiro Yamashita, Susumu Shibasaki, Gentaro Hirokata, Michitaka Ozaki, Satoru Todo, and Kazuo Umezawa.
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Journal Title
Inflammation Research
Volume: 60
Pages: 879-888
Peer Reviewed
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