Development of cardiac regenerative medicine using a novel human somatic stem cell (Muse cell)

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659667
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: Osaka University
• Principal Investigator: SOUGAWA Nagako 大阪大学, 医学系研究科, 特任研究員 (30432579)
• Co-Investigator(Kenkyū-buntansha): SAWA Yoshiki 大阪大学, 大学院・医学系研究科, 教授 (00243220) ; MIYAGAWA Shigeru 大阪大学, 大学院・医学系研究科, 講師 (70544237) ; NISHI Hiroyuki 大阪大学, 大学院・医学系研究科, 助教 (00529208) ; YOSHIKAWA Yasushi 大阪大学, 大学院・医学系研究科, 助教 (40570594) ; IDE Haruki 大阪大学, 大学院・医学系研究科, 特任助教 (90600122)
• Project Period (FY): 2011 – 2012
• Keywords: 再生医学 / 幹細胞 / Muse 細胞

Research Abstract

The purpose of this study is to investigate whether Muse cells can overcome the problems embryonic stem cells and induced pluripotent stem cells have and can contribute to regenerative medicine. Although Muse cells (SSEA3 positive cells) existed in normal human dermal fibroblasts, there were great differences in SSEA3 positive rate among cell lots, so that it was extremely difficult to obtain Muse cells stably, and we could not get the sufficient number of the cells. Therefore, for further investigations, we need the search for the cells and the establishment of maintenance culture methods to obtain the SSEA3 positive cells stably.