Development of new treatment of heart failure using induced pluripotent stem cells-derived cardiomyocytes

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659669
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: Osaka University
• Principal Investigator: NISHI Hiroyuki 大阪大学, 医学系研究科, 助教 (00529208)
• Co-Investigator(Kenkyū-buntansha): SAWA Yoshiki 大阪大学, 大学院・医学系研究科, 教授 (00243220) ; 倉谷 徹 大阪大学, 大学院・医学系研究科, 寄附講座教授 (90448035) ; 宮川 繁 大阪大学, 大学院・医学系研究科, 講師 (70544237) ; 吉川 泰司 大阪大学, 大学院・医学系研究科, 助教 (40570594) ; 福嶌 五月 大阪大学, 大学院・医学系研究科, 助教 (80596867)
• Project Period (FY): 2011 – 2012
• Keywords: iPS 細胞 / 心筋細胞シート / 心筋梗塞 / 心不全

Research Abstract

Induced pluripotent stem cells (iPS cells) of human origin were cultivated and differentiated to cardiomyocytes by originally developed protocol. Cardiomyocyte cell-sheet was then generated from the cardiomyocytes derived from the iPS cells. Transplantation of cardiomyocyte cell-sheets into the infarct heart induced functional integration of the iPS cells-derived cardiomyocytes into the cardiac tissue, contributing to functional recovery, in a rat and a porcine model.

Research Products

• [Journal Article] In vivo differentiation of induced pluripotent stem cell-derived cardiomyocytes (2013)
  • Author(s): Yu T, Miyagawa S, Miki K, Saito A, Fukushima S, Higuchi T, Kawamura M, Kawamura T, Ito E, Kawaguchi N, Sawa Y, Matsuura N
  • Journal Title: Circ J. Volume: 77(5) Pages: 1297-306
  • URL: http://dx.doi.org/10.1253/circj.CJ-12-0977
  • Peer Reviewed
• [Journal Article] Bioengineered myocardium derived from induced pluripote t stem cells n improves cardiac function and attenuates cardiac remodeling following chronic myocardial infarction in rats (2012)
  • Author(s): Miki K, Uenaka H, Saito A, Miyagawa S,Sakaguchi T, Higuchi T, Shimizu T, Okano T, Yamanaka , S SawaY.
  • Journal Title: Stem Cells Transl Med. Volume: 1(5) Pages: 430-7
  • DOI: doi:10.5966/sctm.2011-0038.
  • Peer Reviewed
• [Journal Article] Feasibility, safety, and therapeutic efficacy of human induced pluri ent st cell-derived cardiomyocyte sheets in a porcine ischemic cardiomyopathy model (2012)
  • Author(s): Kawamura M, Miyagawa S,Miki K, Saito A,Fukushima S, Higuchi T, Kawamura T, Kuratani T,Daimon T, Shimizu T, Okano T, Sawa Y.
  • Journal Title: Circulation Volume: 126 Pages: S29-37
  • DOI: doi:10.1161/CIRCULATIONAHA.111.084343
  • Peer Reviewed
• [Presentation] Development of Vascular-Rich Tissue Containing Cardiomyocytes Derived from Human iPS Cells in vivo. (2012)
  • Author(s): Masashi Kawamura
  • Organizer: American Heart Association Scientific Meeting 2012
  • Place of Presentation: ロサンゼルス・コンベンションセンター.米国
  • Year and Date: 20121103-07
• [Presentation] iPS細胞由来心筋前駆細胞シートによる 重症心不全治療の縦断的開発. (2012)
  • Author(s): 福嶌五月
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20120612-14
• [Presentation] ミニブタ虚血性心筋症モデルに対するヒト iPS 細胞由来心筋細胞シートの治療効果の検討 (2012)
  • Author(s): 川村 匡
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20120612-14
• [Presentation] In Vivo Functional Evaluation of Induced Pluripotent Stem Cell-Derived Cardiomyocytes Implanted into the Rat Chronic Infarction Model. (2011)
  • Author(s): Takahiro Higuchi
  • Organizer: American Heart Association Scientific Meeting 2011
  • Place of Presentation: オーランドコンベンションセンター 米国
  • Year and Date: 20111112-16