2013 Fiscal Year Final Research Report
Investigation for tyrosine kinase mutations using novel methods
| Project/Area Number |
23659674
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Thoracic surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
FUJII Yoshitaka 名古屋市立大学, 医学(系)研究科(研究院), 教授 (40156831)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Hidefumi 名古屋市立大学, 大学院医学研究科, 准教授 (00336695)
YANO Motoki 名古屋市立大学, 大学院医学研究科, 准教授 (40315833)
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| Project Period (FY) |
2011 – 2013
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| Keywords | EGFR / RET / NTRK1 / BRAF / CAST-PCR / Immunohistochemistry / V600E |
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| Research Abstract |
Molecular targeted therapies such as crizotinib that target ALK fusion and erlotinib or gefitinib that target EGFR mutations have demonstrated superior single agent activity in selected patients as compared to standard chemotherapy regimes in lung cancer treatment. Recently, a series of new gene fusions have been described in patients with lung cancer associated with the kinase domain of the RET and NTRK1 gene using next generation sequencing. We have developed FISH method to detect RET fusions. In our cohort three RET fusion mutants were found, all were female, non-smoker and adenocarcinomas. Point mutation of the BRAF gene is a genetic event in a subset of lung cancer. In addition, BRAF V600E is a driver mutation that can be effectively targeted with selective BRAF and/or MEK inhibitors. To determine the BRAF mutation status in Japanese lung carcinoma, we investigated BRAF V600E mutations by real time-PCR (CAST-PCR) and immunohistochemical methods using mutation specific antibody.
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[Journal Article] Oncogenic and drug sensitive NTRK1 arrangements in lung cancer2013
Author(s)
Vaishnavi A, Capelletti M, Le AT, Kako S, Butaney M, Ercan D, Mahale S, Davis KD, Aisner DL, Pilling AB, Berge EM, Kim J, Sasaki H, Park S, Haas J, Andrews SW, Lipson D, Stephens PJ, Miller VA, Varcella-Garcia M, Janne PA, Doebele RC
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Journal Title
Nat Med
Volume: 19
Pages: 1469-1472
DOI
Peer Reviewed
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[Journal Article] RET expression and detection of KIF5B/RET gene rearrangements in Japanese lung cancer2012
Author(s)
Sasaki H, Shimizu S, Tani Y, Maekawa M, Okuda K, Yokota K, Shitara M, Hikosaka Y, Moriyama S, Yano M, Fujii Y
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Journal Title
Cancer Med
Volume: 1(1)
Pages: 68-75
DOI
Peer Reviewed
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[Journal Article] Identification of new ALK and RET gene fusions from colorectal and lung cancer biopsies2012
Author(s)
Lipson D, Capelletti M, Yelensky R, Otto G, Parker A, Jarosz M, Curran JA, Balasubramanian S, Bloom T, Brennan KW, Donahue A, Downing SR, Frampton GM, Garcia L, Juhn F, Mitchell KC, White E, White J, Zwirko Z, Peretz T, Nechushtan H, Soussan-Gutman L, Kim J, Sasaki H, Kim HR, Park SI, Ercan D, Sheehan CE, Ross JS, Cronin MT, Janne PA, Stephens PJ
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Journal Title
Nat Med
Volume: 18(3)
Pages: 382-384
DOI
Peer Reviewed
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