2013 Fiscal Year Final Research Report
Isolation and characterization of an N-linked oligosaccharide that is increased in primary central nervous system lymphoma tissue and cell lines.
| Project/Area Number |
23659695
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
YAMANAKA RYUYA 京都府立医科大学, 医学部, 教授 (20323991)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IKEAKA Kazuhiro 自然科学研究機構, 生理学研究所, 教授 (00144527)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 中枢神経原発悪性リンパ腫 / 糖タンパク糖鎖 |
|---|
| Research Abstract |
We have isolated and characterized N-linked oligo-saccharides that are significantly increased in primary central nervous system lymphoma (PCNSL) tissue and cell lines. The structures of N-linked oligosaccharides present in 3 human normal brain tissues, 34 patients with PCNSL and 1 PCNSL cell lines were analyzed by partially automated technique for the isolation and fluorescent labeling of N-linked sugar chains from glycoproteins. Characterization of the sugar chains was achieved with the use of a combination of HPLC columns and a highly sensitive fluorescence detector at femtomole levels. By collecting peaks which accounted for 0.1% or more, twenty-one different oligosaccharide structures were characterized. The amount of specific structure for PCNSL is now under investigation. Oligo-saccharides in specimens may provide a novel marker and target for the diagnosis and treatment of PCNSL, respectively.
|
-
-
-
[Journal Article] Phase II study of personalized peptide vaccination for refractory bone and soft tissue sarcoma patients2013
Author(s)
Takahashi R, Ishibashi Y, Hiraoka K, Matsueda S, Kawano K, Kawahara A, Kage M, Ohshima K, Yamanaka R, Shichijo S, Shirouzu K, Itoh K, Sasada T
-
Journal Title
Cancer Sci
Volume: 104(10)
Pages: 1285-1294
-
-
[Journal Article] Identification and Validation of a Gene Expression Signature That Predicts Outcome in Malignant Glioma Patients2012
Author(s)
Kawaguchi A, Yajima N, Komohara Y, Aoki H, Tsuchiya N, Homma J, Sano M, Natsumeda M, Uzuka T, Saitoh A, Takahashi H, Sakai Y, Takahashi H, Fujii Y, Kakuma T, Yamanaka R
-
Journal Title
Int J Oncol
Volume: 40(3)
Pages: 721-730
-
[Journal Article] Gene expression signature-based prognostic risk score in patients with primary central nervous system lymphoma2012
Author(s)
Kawaguchi A, Iwadate Y, Komohara Y, Sano M, Kajiwara K, Yajima N, Tsuchiya N, Homma J, Aoki H, Kobayashi T, Sakai Y, Hondoh H, Fujii Y, Kakuma T, Yamanaka R
-
Journal Title
Clin Cancer Res
Volume: 18(20)
Pages: 5672-5681
-
-
[Journal Article] Assessment of immunological biomarkers in patients with advanced cancer treated by personalized peptide vaccination2011
Author(s)
Noguchi M, Mine T, Komatsu N, Suekane S, Moriya F, Matsuoka K, Yutani S, Shichijo S, Yamada A, Toh U, Kawano K, Azuma K, Uemura H, Okuno K, Matsumoto K, Yanagimoto H, Yamanaka R, Oka M, Todo S, Sasada T, Itoh K
-
Journal Title
Cancer Biol Ther
Volume: 10(12)
Pages: 1266-1279
-
[Journal Article] M2 macrophage/microglial cells induce activation of stat3 in primary central nervous system lymphoma2011
Author(s)
Komohara Y, Horlad H, Ohnishi K, Ohta K, Makino K, Hondo H, Yamanaka R, Kajiwara K, Saito T, Kuratsu J, Takeya M
-
Journal Title
J Clin Exp Hematop
Volume: 51(2)
Pages: 93-99
-
[Journal Article] 外来化学療法センターにおける症状チエックシートの有用2011
Author(s)
吉田直久,中村晃和,松田清美,菅谷和子,富田英津子,石川 剛,八木信明,古倉 聡,内藤裕二,山中龍也,谷脇雅史,吉川敏一,三木恒治
-
Journal Title
Progress in Medicine
Volume: 31(4)
Pages: 1163-1168
-
-
-
-
-
-
-
-
-
-
-
-
-
