2012 Fiscal Year Final Research Report
A study of bone atrophy treatment by detection of factors in osteocytes involved in mechanical stress
| Project/Area Number |
23659717
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 骨細胞 / メカノセンサー / イオンチャネル |
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| Research Abstract |
Genome-wide screening using osteocytes in disused bone showed upregulation of BK channel genes. RT-PCR revealed that osteocytes express KCNMB1, 2 and 4 subunit genes. CaK-GFP transgenic mice were generated, and then Col1a1-mRFP1; CaK-GFP double hemi-transgenic mice were produced. We are trying live imaging of osteoblasts and osteclasts in these mice using confocal microscope when BK channel opener or blocker reagents are administered.
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