2012 Fiscal Year Final Research Report
Development of the novel compound which specifically scavenges singlet oxygen extra- and intracellularly.
| Project/Area Number |
23659740
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Kouhei 京都大学, 医学研究科, 講師 (80402858)
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| Co-Investigator(Renkei-kenkyūsha) |
ENDO Nobuyuki 若狭湾エネルギー研究センター, 研究開発部, 主査研究員 (30359244)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 一重項酸素 / 特異的消去剤 / 神経細胞 / 皮膚細胞 / 細胞死 / 放射線障害 |
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| Research Abstract |
Dimethyl-hydropterin (DPH) was newly synthesized through the reaction with pterin and edaravone. The direct analysis of near-infrared luminescence using Raman spectroscopy proved that DPH quenched the photochemically generated singlet oxygen in a cell-free system. Further, the chemiluminescence assay revealed that DPH scavenged not only singlet oxygen but also superoxide generated in activated neutrophils. When singlet oxygen was generated photochemically in neuronal cells, cell death was induced. DPH prevented the singlet oxygen-induced cell death. And, when keratinocytes was weakly exposed to the ionized radiation, cell death was not observed but the DNA damage was observed. DPH attenuated the DNA damage, probably due to scavenging singlet oxygen.
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