2012 Fiscal Year Final Research Report
A new protective method of post transplantation lung fibrosis with TGF beta signal pathway regulation
| Project/Area Number |
23659741
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 周術期管理学 |
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| Research Abstract |
In cell culture, we showed that TGF-βstimulation induced TGF-βsignal proteins, such as Smad2/3, TSP-1 in A549 cells by western blotting method. This development of TGF-β signal proteins by TGF-βstimulation in A549 cells inhibited by halofuginone in dose dependent manner. In animal study, at first, we tried tomake rat lung transplantation models. Unexpectedly it is too difficult to establish lung transplantation in such small animals. Next swine transplantation models were planned. Before lung transplantation surgery of swine, I became proficient in general anesthesia and various vital monitoring and surgical procedure of swine.
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