Proteomic analysis of lung epithelial lining fluids collected from the patients suffering from acute lung injury.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23659748
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SAWA Teiji 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10206013)
• Co-Investigator(Kenkyū-buntansha): IKEGAWA Masaya 同志社大学, 生命科学, 教授 (60381943) ; HASHIMOTO Satoru 京都府立医科大学, 医学研究科, 病院教授 (90167578) ; MATSUYAMA Hiroki 京都府立医科大学, 医学研究科, 客員講師 (80515289)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 急性肺傷害 / 気管支鏡下マイクロサンプリング法 / プロテオーム / 肺上皮被覆液 / LCMSMS / ELFICAT

Outline of Final Research Achievements

We established the new method for the proteomic analysis of the lung epithelial lining fluids. In this study, we harvested the lung epithelial lining fluids by using bronchoscopic micro sampling (BMS), and analyzed them by LC/MS/MS. This method enabled us to efficiently and effectively analyze the micro volume samples, in comparison with classic methods such as bronchoalveolar lavage (BAL). To analyze the proteomic data by web-browser based computer technology, we developed a new analysis software named “Elficat”. Elficat uses various software technologies such as XML-database, and sever-side XSLT and SVG. By using the Elficat, we can visualize the data from multiple groups that contain the data from a set of patients. We can apply this method to the proteomic analysis for acute lung injury such as acute respiratory distress syndrome.

Free Research Field

集中治療医学