2012 Fiscal Year Final Research Report
Study on the molecules responsible for implantation window
| Project/Area Number |
23659767
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
SUZUKI Hiroshi 帯広畜産大学, 原虫病研究センター, 教授 (60333473)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 着床 / 胚 / 遺伝子 / 妊娠 / 偽妊娠 |
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| Research Abstract |
To develop a control method for embryonic implantation and subsequent differentiation and proliferation as well as recognition of embryonic attachment in uterus, expression pattern of genes responsible for initiation of implantation window in uterine tissues from an identical recipient which transferred fertilized oocytes at pronucleus stage and embryos at 8-cell stage into right and left side of the oviduct, respectively, was examined by using a DNA microarray. As a result, at the open of implantation window, one down-regulated and two up-regulated genes were identified. These presumably responsible genes for window of implantation were further analyzed the expression profile during the course of preimplantation development and implantation by a real time PCR method. Expression of Sprr2f was highest on 0.5 dpc and gradually decreased with preimplantation development and during implantation. However, Sprr2f strongly expressed in ovarian tissues on 3.5 dpc. Ass1 highly expressed in uterus on 4.5 dpc and in ovary on 3.5 dpc. Further analysis of these molecules as candidates of genes responsible for regulation of implantation window is required in uterine and ovarian tissues at peri-implantation period.
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Research Products
(18 results)
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[Journal Article] Cryopreservation of canine embryos2011
Author(s)
Abe, Y., Suwa, Y., Asano, T., Ueta, Y. Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K., Suzuki, H.
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Journal Title
Biol. Reprod
Volume: 84
Pages: 363-368
Peer Reviewed
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[Presentation] Puppies from frozen embryos2011
Author(s)
Suzuki, H.
Organizer
International Meeting for Evolution of Reproductive Biology and Task of Frontiers: Trajectory and Prospective of IVF, Stem Cell and Epigenetic Study
Place of Presentation
AIINA Hall, Morioka, Iwate, Japan.
Year and Date
20110913-17
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