Study on the molecules responsible for implantation window

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659767
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: SUZUKI Hiroshi 帯広畜産大学, 原虫病研究センター, 教授 (60333473)
• Project Period (FY): 2011 – 2012
• Keywords: 着床 / 胚 / 遺伝子 / 妊娠 / 偽妊娠

Research Abstract

To develop a control method for embryonic implantation and subsequent differentiation and proliferation as well as recognition of embryonic attachment in uterus, expression pattern of genes responsible for initiation of implantation window in uterine tissues from an identical recipient which transferred fertilized oocytes at pronucleus stage and embryos at 8-cell stage into right and left side of the oviduct, respectively, was examined by using a DNA microarray. As a result, at the open of implantation window, one down-regulated and two up-regulated genes were identified. These presumably responsible genes for window of implantation were further analyzed the expression profile during the course of preimplantation development and implantation by a real time PCR method. Expression of Sprr2f was highest on 0.5 dpc and gradually decreased with preimplantation development and during implantation. However, Sprr2f strongly expressed in ovarian tissues on 3.5 dpc. Ass1 highly expressed in uterus on 4.5 dpc and in ovary on 3.5 dpc. Further analysis of these molecules as candidates of genes responsible for regulation of implantation window is required in uterine and ovarian tissues at peri-implantation period.

Research Products

• [Journal Article] Sexing of dog sperm by fluorescence in situ hybridization (2013)
  • Author(s): Oi, M., Yamada, K., Hayakawa, H., Suzuki, H.
  • Journal Title: J. Reprod. Dev Volume: 59 Pages: 92-96
  • Peer Reviewed
• [Journal Article] Beneficial effect of hyperbaric oxygen therapy on the follicular survival in the mouse ovary after transplantation (2012)
  • Author(s): Hayashi, S., Asano, T., Kakizaki, R., Suzuki, H.
  • Journal Title: J. Reprod. Dev Volume: 58 Pages: 260-263
  • Peer Reviewed
• [Journal Article] In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers (2012)
  • Author(s): Abdel-Ghani, M., Shimizu, T., Asano, T., Suzuki, H.
  • Journal Title: Theriogenology Volume: 77 Pages: 347-355
  • Peer Reviewed
• [Journal Article] Cryopreservation of canine embryos and resulting pregnancies. (2012)
  • Author(s): Suzuki, H.
  • Journal Title: Reprod. Dom. Anim Volume: 47 Pages: 141-143
  • Peer Reviewed
• [Journal Article] Effect of bovine cumulus-oocyte complexes-conditioned medium on in vitro maturation of canine oocytes. Reprod (2011)
  • Author(s): Abdel-Ghani, M., Abe, Y., Asano, T., Hamano, S., Suzuki, H.
  • Journal Title: Med. Biol Volume: 10 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Cryopreservation of canine embryos (2011)
  • Author(s): Abe, Y., Suwa, Y., Asano, T., Ueta, Y. Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K., Suzuki, H.
  • Journal Title: Biol. Reprod Volume: 84 Pages: 363-368
  • Peer Reviewed
• [Journal Article] A study on freeze-drying as a method of preserving mouse sperm. (2011)
  • Author(s): Kawase, Y., Suzuki, H.
  • Journal Title: J. Reprod. Dev Volume: 57 Pages: 176-182
  • Peer Reviewed
• [Journal Article] Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice (2011)
  • Author(s): Abdel-Ghani, M., Abe, Y., Asano, T., Suzuki, H.
  • Journal Title: Reprod. Med. Biol Volume: 10 Pages: 259-266
  • Peer Reviewed
• [Journal Article] Effect of intraperitoneal administration of desialylated erythropoietin on the follicular survival in cryopreserved canine ovaries after xenotransplantation (2011)
  • Author(s): Hirayama, Y., Inoue, K., Suzuki, H.
  • Journal Title: J.Mamm. Ova Res Volume: 28 Pages: 143-147
  • Peer Reviewed
• [Presentation] マラリア原虫感染が妊娠時特異的に重篤化する免疫学的メカニズムの解明 (2012)
  • Author(s): 新田あかね、白砂孔明、松本茜、鈴木宏志
  • Organizer: 第105回日本繁殖生物学会
  • Place of Presentation: 筑波大学会館、つくば市
  • Year and Date: 20120905-08
• [Presentation] Analysis of the immunological mechanisms that malaria leads to a pregnancy-specific severity (2012)
  • Author(s): Nitta, A., Shirasuna, K., Matsumoto, A., Suzuki, H.
  • Organizer: 45th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: State College, Pennsylvania, USA.
  • Year and Date: 20120812-15
• [Presentation] Sperm Sexing in the dog by fluorescence in situ hybridization (2012)
  • Author(s): Suzuki, H., Oi, M.
  • Organizer: 45th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: State College, Pennsylvania, USA.
  • Year and Date: 20120812-15
• [Presentation] Effect of hyperbaric oxgen therapy on the follicular reserve on canine ovarian tissues after xenotransplantation (2012)
  • Author(s): Suzuki, H.
  • Organizer: 17th International Congress on Animal Reproduction
  • Place of Presentation: Vancouver, British Columbia, Canada.
  • Year and Date: 20120729-0802
• [Presentation] Puppies from liquid nitrogen -Cryopreservation of canine embryos- (2012)
  • Author(s): Suzuki, H.
  • Organizer: 7th International Symposium on Canine and Feline Reproduction
  • Place of Presentation: Whistler, British Columbia, Canada
  • Year and Date: 20120726-29
• [Presentation] Puppies from frozen embryos (2011)
  • Author(s): Suzuki, H.
  • Organizer: International Meeting for Evolution of Reproductive Biology and Task of Frontiers: Trajectory and Prospective of IVF, Stem Cell and Epigenetic Study
  • Place of Presentation: AIINA Hall, Morioka, Iwate, Japan.
  • Year and Date: 20110913-17
• [Presentation] Cryopreservation of canine embryos (2011)
  • Author(s): Suzuki, H.
  • Organizer: The 12th International Congress on Reproductive Biomedicine
  • Place of Presentation: Royan Institute, Tehran, The Islamic Republic of Iran
  • Year and Date: 20110907-09
• [Presentation] Follicular loss of cryopreserved canine ovary after xenotransplantation and its solution (2011)
  • Author(s): Suzuki, H.
  • Organizer: The 12th International Congress on Reproductive Biomedicine
  • Place of Presentation: Royan Institute, Tehran, The Islamic Republic of Iran
  • Year and Date: 20110907-09
• [Presentation] Effect of hyperbaric oxygen therapy on the follicular loss of ovarian tissues after transplantation (2011)
  • Author(s): Suzuki, H., Hayashi, H., Asano, T.
  • Organizer: 44th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Portland, Oregon, USA.
  • Year and Date: 20110731-0804