Comparative analysis of stem cells between normal and neoplastic myometric cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 23659776
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Shinshu University
• Principal Investigator: SANO Kenji 信州大学, 学術研究院医学系(医学部附属病院), 講師 (50205994)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Takuma 信州大学, 学術研究院医学系, 准教授 (60359726)
• Co-Investigator(Renkei-kenkyūsha): KONISHI Ikuo 京都大学, 学術研究院医学系, 教授 (90192062) ; SHIOZAWA Tanri 信州大学, 学術研究院医学系, 教授 (20235493) ; HORIUCHI Akiko 信州大学, 医学部附属病院, 特任研究員 (80334895)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: stem cell / leiomyoma / leiomyosarcoma / Side Population(SP) / non-SP

Outline of Final Research Achievements

Comparative expression analysis of stem cell-rich polulation was made between normal myometrium and myometrial tumors including uterus leiomyoma and leiomyosarcoma. Side population (SP) of leiomyosarcoma rich in stem cell demonstrated slightly high expression of Glut-1 and HKII, and low expression of ER, PGR, calponin, SMA, MKI-67, Cyclin E compared to the equivalent of normal myometrium and leimyoma. SP of leiomyoma showed slightly low expression of ER, PGR, SMA, calponin compared to normal myometrium. And SP of leiomyosarcoma compared to non-SP showed high expression of ABCG and CD34.These results suggest SP cells of leiomyosarcoma include undifferentiated cell-rich population, and Glut-1 and HKII are possible markers for stem cells.

Free Research Field

分子病理学