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2012 Fiscal Year Final Research Report

Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device

Research Project

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Project/Area Number 23659790
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionTohoku University

Principal Investigator

WADA Hiroshi  東北大学, 大学院・工学研究科, 教授 (30111264)

Co-Investigator(Kenkyū-buntansha) MURAKOSHI Michio  東北大学, 大学院・工学研究科, 助教 (70570901)
KOYAMA Shin  東北大学, 大学院・工学研究科, 助教 (10465487)
KOBAYSHI Toshimitsu  東北大学, 大学院・医学系研究科, 教授 (80133958)
Project Period (FY) 2011 – 2012
Keywords耳科学
Research Abstract

A membrane potential control device, which induces the conformational changes of prestin expressed in the plasma membrane, has been newly developed. Microstructures and electrophysiological properties of the prestin-expressing culture cells and the prestin-reconstituted lipid bilayer were measured by using this device. As a result, it was suggested that the conformational changes of proteins in the plasma membrane may possibly be visualized when the membrane potential is controlled using the developed device. Based on the findings obtained in this study, further research to visualize the conformational changes of prestin reconstituted in the lipid bilayer will be performed using this device.

  • Research Products

    (2 results)

All 2013

All Presentation (2 results)

  • [Presentation] Evaluation of electrophysiological properties of the motor protein prestin expressed in the inner ear2013

    • Author(s)
      Ogawa Y, Murakoshi M, Wada H
    • Organizer
      The 6th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      Singapore
    • Year and Date
      20130323-34
  • [Presentation] 内耳に発 現する膜タンパク質プレスチンの電気 生理学的特性計測のための平面パッチ クランプシステムの開発(Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear)2013

    • Author(s)
      小川雄大,村越道生,和田仁
    • Organizer
      日本機械学会第25回バイ オエンジニアリング講演会
    • Place of Presentation
      筑波
    • Year and Date
      20130109-11

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Published: 2014-09-25  

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