Artificial sight produced by transducing genes into primary visual cortex

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659804
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Iwate University (2012); Tohoku University (2011)
• Principal Investigator: TOMITA Hiroshi 岩手大学, 工学部, 教授 (40302088)
• Co-Investigator(Kenkyū-buntansha): SUGANO Eriko 岩手大学, 工学部, 特任准教授 (70375210) ; 伊藤 拓哉 東北大学, 医学(系)研究科(研究院), 助教 (70396539)
• Project Period (FY): 2011 – 2012
• Keywords: 視覚再生 / 人工網膜 / チャネルロドプシン-2

Research Abstract

The transduction efficiency to the cortex using AAV type 2/5 was higher than that using AAV2/2. However we could not succeed to transfer to the broad area. It was limited to the only injection site. We tested two types of AAVs including a CAG or CaMKII promoter. Different light responsivities elicited by ChR2 transferred cortex were recorded depending on the promoter the AAV included. These results indicated that it is important to choose appropriate promoter to transfer a gene into the cortex.

Research Products

• [Journal Article] Optogenetically Induced Seizure and the Longitudinal Hippocampal Network Dynamics (2013)
  • Author(s): Osawa S, Iwasaki M, Hosaka R, Matsuzaka M, Tomita H, Ishizuka T, Sugano E, Okumura E, Yawo H, Nakasato N, Tominaga T, Mushiake H
  • Journal Title: PUBLIC LIBRARY OF SCIENCE ONE Volume: 8(4)
  • Peer Reviewed
• [Journal Article] Different anti-oxidant effects of thioredoxin 1 and thioredoxin 2 in retinal epithelial cells (2013)
  • Author(s): Sugano E, Isago H, Murayama N, Tamai M, Tomita H.
  • Journal Title: Cell structure and Function Volume: 38(1) Pages: 81-88
  • Peer Reviewed
• [Journal Article] Intravitreal injection or topical eye-drop application of a μ-calpain C2L domain peptide protects against photoreceptor cell death in Royal College of Surgeons' rats, a model of retinitis pigmentosa (2012)
  • Author(s): Ozaki T, Nakazawa M, Yamashita T, Sorimachi H, Hata S, Tomita H, Isago H, Baba A, Ishiguro SI
  • Journal Title: Biochimica et Biophysica Acta Volume: 1822(11) Pages: 1783-1795
  • Peer Reviewed
• [Journal Article] Notch signaling pathway regulates proliferation and differentiation of immortalized Muller cells under hypoxic conditions in vitro (2012)
  • Author(s): Wang Z, Sugano E, Isago H, Hiroi T, Tamai M, Tomita H.
  • Journal Title: Neuroscience Volume: 214(12) Pages: 171-180
  • Peer Reviewed
• [Journal Article] Age-dependent differences in recovered visual responses in Royal College of Surgeons rats transduced with the Channelrhodopsin-2 gene (2012)
  • Author(s): Isago H, Sugano E, Wang Z, Murayama N, Tamai M, Tomita H.
  • Journal Title: Journal of Molecular. Neuroscience Volume: 46(2) Pages: 393-400
  • Peer Reviewed
• [Journal Article] Differentiation of neuronal cells from NIH/3T3 fibroblasts under defined conditions (2011)
  • Author(s): Wang Z, Sugano E, Isago H, Hiroi T, Tamai M, Tomita H.
  • Journal Title: Development Growth and Differentiation Volume: 53(3) Pages: 357-365
  • Peer Reviewed
• [Presentation] チャネルロドプシンを利用した遺伝子治療研究 (2012)
  • Author(s): 菅野江里子
  • Organizer: 日本臨床視覚電気生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-06
• [Presentation] カニクイザルの片眼視細胞変性モデルの確立 (2012)
  • Author(s): 砂金ひとみ,菅野江里子,村山奈美枝,原富雄,萩森一郎,玉井信,富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 変異型チャネルロドプシンのパッチクランプ法による評価 (2012)
  • Author(s): 村山奈美枝,菅野江里子,砂金ひとみ,斎藤建彦,玉井信,富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] Establishment of a method for the visual acuity test on cynomolgus monkey. XIVth International Symposium on Retinal Degeneration (2012)
  • Author(s): Tomita H, Isago H, Iwata E, Murayama N, Shinomoto Y. Watanabe M, Tamai M, Sugano E.
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology
  • Place of Presentation: Ft. Lauderdale, FL.
  • Year and Date: 2012-05-09
• [Presentation] Restore vision covering visible light by using single gene, modified volvox channelrhodopsin-1 (2012)
  • Author(s): Sugano E, Isago H, Murayama N, Saitoh T, Tamai M, Tomita H.
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology
  • Place of Presentation: Ft. Lauderdale, FL.
  • Year and Date: 2012-05-07
• [Presentation] 遺伝治療による視覚再生 (2012)
  • Author(s): 富田浩史
  • Organizer: 近畿眼科先進医療研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-02-04
• [Presentation] 緑藻類チャネルロドプシン遺伝子を用いた視覚再生研究:現状と課題 (2011)
  • Author(s): 富田浩史
  • Organizer: 日本網膜色素変性症協会(JRPS)岩手県支部総会・医療講演会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-09-10
• [Presentation] Visual properties of genetically blind rats transduced with channelrhodopsin-2 gene (2011)
  • Author(s): Tomita H
  • Organizer: The 4th Global Chinese Ophthalmic Conference
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2011-09-08
• [Presentation] 遺伝子治療による視覚再建 ～ 緑藻類由来遺伝子の利用 ～ (2011)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山奈美枝, 玉井信
  • Organizer: 第17回バーチャルリアリティ心理学研究会
  • Place of Presentation: 富山(国際会議場)
  • Year and Date: 2011-07-23
• [Presentation] 緑藻類チャネルロドプシン遺伝子を用いた視覚再生研究:現状と課題 (2011)
  • Author(s): 富田浩史
  • Organizer: 日本網膜色素変性症協会(JRPS)三重県支部総会・医療講演会
  • Place of Presentation: 松阪
  • Year and Date: 2011-06-21
• [Presentation] 緑藻類チャネルロドプシン遺伝子を用いた視覚再生研究:現状と課題 (2011)
  • Author(s): 富田浩史
  • Organizer: 日本網膜色素変性症協会(JRPS)福岡県支部総会・医療講演会
  • Place of Presentation: 福岡
  • Year and Date: 2011-06-05
• [Presentation] 視覚再生研究の新たな展開 (2011)
  • Author(s): 日富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山奈美枝, 玉井信
  • Organizer: 日本眼科学会総会イヴニングセミナー
  • Place of Presentation: 東京(東京国際フォーラム)
  • Year and Date: 2011-05-13
• [Presentation] Effective Time Point For Channelrhodopsin-2 mediated Gene Therapy After Photoreceptor Degeneration In RCS Rats (2011)
  • Author(s): Sugano E, Tomita H, Isago H, Hiroi T, Wang Z. Tamai M.
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Ft. Lauderdale
  • Year and Date: 2011-05-01
• [Book] オプトジェネティクス(光遺伝学)～光工学と遺伝学による行動制御技術の最前線～「チャネルロドプシンによる遺伝子治療の開発」 (2013)
  • Author(s): 菅野江里子、富田浩史
  • Total Pages: 248-257
  • Publisher: (株)エヌ・ティー・エス出版
• [Book] Gene Therapy for Retinitis pigmentosa, Gene Therapy / Book 2 (2012)
  • Author(s): Tomita H, Sugano E, Isago H, Tamai M.
  • Total Pages: 494-507
  • Publisher: InTech Books
• [Book] 【網膜の視覚再生】 わかりやすい臨床講座 チャネルロドプシンを用いた視覚再生、日本の眼科 (2011)
  • Author(s): 富田浩史、菅野江里子
  • Total Pages: 1602-1607
  • Publisher: 社団法人日本眼科医会出版
• [Book] 「遺伝子治療による新たな視覚再生法 チャネルロドプシン遺伝子導入が鍵」, Medical Tribune (2011)
  • Author(s): 富田浩史
  • Total Pages: 48
  • Publisher: メディカルトリビューン社発行
• [Remarks] 岩手大学工学部
  • URL: http://www.eng.iwate-u.ac.jp/jp/profile/tomita_hiroshi.html
• [Remarks] 東北大学臨床試験推進センター
  • URL: http://www.crieto.hosp.tohoku.ac.jp/seedlist/seed14.html
• [Remarks] 東北大学脳科学センター
  • URL: http://www.bsc.tohoku.ac.jp/contents/c2_32/c2_32_tomita_h.html