The diagnostic and therapeutic strategies for age-related macular degeneration and glaucoma targeting the amyloid b

2013 Fiscal Year Final Research Report

• Project/Area Number: 23659808
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OHNO-MATSUI Kyoko 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (30262174)
• Co-Investigator(Kenkyū-buntansha): MORITA Ikuo 東京医科歯科大学, 医歯学総合研究科, 教授 (60100129)
• Project Period (FY): 2011 – 2012
• Keywords: 加齢黄斑変性 / Amyloid β / 緑内障 / ラマン分光

Research Abstract

We previously demonstrated that Alzheimer's amyloid beta(Ab) was an important causative material for age-related macular degeneration (AMD). Recent studies showed Ab accumulation within retina in glaucoma. These suggest that Ab deposition might be a common precursors for both pathologies. To develop the methods of in vivo visualization of Ab deposited in the retina, we injected Ab into subretinal space of mouse eyes and injected the PiB; via tail vein. In retinal flatmount, subretinal Ab was clearly observed. However, the amount of Ab in the retina was not sufficient to be detected in neprilysin deficient mice. We also performed Raman spectroscopy to detect Ab in a non-invasive way.

Research Products

• [Journal Article] Analyses of Shape of Eyes and Structure of Optic Nerves in eyes with Tilted Disc Syndrome by Swept-source Optical Coherence Tomography and Three Dimensional Magnetic Resonance Imaging (2013)
  • Author(s): Shinohara K, Moriyama M, Shimada N, Nagaoka N, Ishibashi T, Tokoro T, Ohno-Matsui K
  • Journal Title: Eye Volume: 27(11) Pages: 1233-1241
  • Peer Reviewed
• [Journal Article] Cholesterol enhances amyloid β deposition in mouse retina by modulating the activities of Aβ-regulating enzymes in retinal pigment epithelial cells (2012)
  • Author(s): Wang J, Ohno-Matsui K, Morita I
  • Journal Title: Biophys Biochem Res Commun Volume: 424 Pages: 704-709
  • Peer Reviewed
• [Journal Article] Elevated amyloid β production in senescent retinal pigment epithelium, a possible mechanism of age-related subretinal accumulation of amyloid β (2012)
  • Author(s): Wang J, Ohno-Matsui K, Morita I
  • Journal Title: Biochem Biophys Res Commun Volume: 423 Pages: 73-78
  • Peer Reviewed
• [Journal Article] Amyloid β. enhances migration of endothelial progenitor cells by up-regulating CX3CR1 in response to fractalkine, which may be associated with development of choroidal neovascularization (2011)
  • Author(s): Wang J, Ohno-Matsui K, Nakahama K, Okamoto A, Yoshida T, Shimada N, Mochizuki M, Morita I
  • Journal Title: Arteriosclerosis, Thrombosis, and Vascular Biology Volume: 31 Pages: e11-18
  • Peer Reviewed
• [Presentation] Amyloid beta enhances migration of endothelial progenitor cells via upregulation of CX3CR1 (2012)
  • Author(s): Jiying Wang, Kyoko Ohno-Matsui, Takeshi Yoshida, Noriaki Shimada, Manabu Mochizuki, Ikuo Morita
  • Organizer: American Association of Research of Vision and Ophthalmology (ARVO)
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2012-05-03
• [Presentation] The potential role of amyloid β in the development of choroidal neovascularization in age-relaed macular degeneration. Syymposium "Fundamental mechanism underlying both physiological and pathological angiogenesis" (2011)
  • Author(s): Kyoko Ohno-Matsui
  • Organizer: The 19th Annual Meeting of the Japanese Vascular Biology and Medicine Organization and the 1st Asia-Pacific Vascular Biology Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2011-12-09
• [Presentation] Amyloid β enhances the migration of endothelial progenitor cells via CX3CR1 (2011)
  • Author(s): Kyoko Ohno-Matsui
  • Organizer: 5th International Symposium on Age-Related Macular Degeneration
  • Place of Presentation: Baden Baden, Germany
  • Year and Date: 2011-09-09
• [Presentation] Amyloid beta enhances migration of endothelial progenitor cells by up-regulating CX3CR1 and stimulates development of choroidal neovascularization. In symposium "Advaces on molecular mechanisms in intraocular inflammation, neovascularization and neuroprotection" (2011)
  • Author(s): Kyoko Ohno-Matsui
  • Organizer: Asia Pacific Academy of Ophthalmology Congress 2011
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2011-03-24