2012 Fiscal Year Final Research Report
Investigation of Acanthamoeba infection mechanism in cornea
| Project/Area Number |
23659812
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Ehime University |
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Principal Investigator |
SAKANE Yuri 愛媛大学, 大学院・医学系研究科, 寄附講座助教 (00601478)
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| Co-Investigator(Kenkyū-buntansha) |
OHASHI Yuichi 愛媛大学, 大学院・医学系研究科, 教授 (00116005)
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| Project Period (FY) |
2011 – 2012
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| Keywords | アカントアメーバ / 角膜上皮 / MBP1 / 蛍光標識 / MALDI-TOF/MS |
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| Research Abstract |
In this study, we investigated receptor for Mannose-binding protein(MBP1) which thought to be need for establishment of acanthamoeba keratitis. We made MBP1 chimera protein by cell free system and analyzed binding protein with the MBP1 chimera protein by MALDI-TOF/MS. Also we made ubiquitin promoter fluorescent protein constructs, and then we made fluorescent protein labeled acanthamoeba.
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Research Products
(36 results)
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[Journal Article] Important role of epiregulin in inflammatory responses during corneal epithelial wound healing.2012
Author(s)
Zhang, Y., Kobayashi, T., Hayashi, Y., Yoshioka, R., Shiraishi, A., Shirasawa, S., Higashiyama, S., Ohashi, Y.
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Journal Title
Invest Ophthalmol Vis Sci
Volume: 53
Pages: 2414-2423
Peer Reviewed
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[Journal Article] Involvement of stem cell factor and c-kit in corneal wound healing in mice2012
Author(s)
Miyamoto, K., Kobayashi, T., Hayashi, Y., Zhang, Y., Hara, Y., Higashine, M., Shiraishi, A., Ohashi, Y.
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Journal Title
Mol Vis
Volume: 18
Pages: 1505-1515
Peer Reviewed
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[Journal Article] Assessment of real-time polymerase chain reaction detection of Acanthamoeba and prognosis determinants of Acanthamoeba keratitis2012
Author(s)
Ikeda, Y., Miyazaki, D., Yakura, K., Kawaguchi, A., Ishikura, R., Inoue, Y., Mito, T., Shiraishi, A., Ohashi, Y., Higaki, S., Itahashi, M., Fukuda, M., Shimomura, Y., Yagita, K.
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Journal Title
Ophthalmology
Volume: 119
Pages: 1111-1119
Peer Reviewed
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[Journal Article] Anterior segment optical coherence tomography analysis of clinically unilateral pseudoexfoliation syndrome: evidence of bilateral involvement and morphologic factors related to asymmetry2011
Author(s)
Zheng, X., Sakai, H., Goto, T., Namiguchi, K., Mizoue, S., Shiraishi, A., Sawaguchi, S., Ohashi, Y.
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Journal Title
Invest Ophthalmol Vis Sci
Volume: 52
Pages: 5679-5684
Peer Reviewed
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[Journal Article] In vivo confocal microscopic evidence of keratopathy in patients with pseudoexfoliation syndrome.2011
Author(s)
Zheng, X., Shiraishi, A., Okuma, S., Mizoue, S., Goto, T., Kawasaki, S., Uno, T., Miyoshi, T., Ruggeri, A., Ohashi, Y.
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Journal Title
Invest Ophthalmol Vis Sci
Volume: 52
Pages: 1755-1761
Peer Reviewed
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[Journal Article] Lumican is required for neutrophil extravasation following corneal injury and wound healing.2010
Author(s)
Hayashi Y, Call MK, Chikama T, Liu H, Carlson EC, Sun Y, Pearlman E, Funderburgh JL, Babcock G, Liu CY, Ohashi Y, Kao W.
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Journal Title
Journal of Cell Science
Volume: 123
Pages: 2987-2995
Peer Reviewed
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