2013 Fiscal Year Final Research Report
Controlled release of granulocyte colony-stimulating factor (G-CSF) enhances osteoconductive and biodegradable properties of beta-tricalcium phosphate (beta-TCP) in a rat calvarial defect model
| Project/Area Number |
23659826
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
OYAMA Akihiko 北海道大学, 大学病院, 講師 (70374486)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Yuhei 北海道大学, 大学院医学研究科, 教授 (70271674)
FURUKAWA Hiroshi 北海道大学, 大学院医学研究科, 講師 (00399924)
FUNAYAMA Emi 北海道大学, 大学院医学研究科, 助教 (10533630)
HAYASHI Toshikhiko 北海道大学, 大学院歯学研究科, 准教授 (00432146)
SAITO Akira 北海道大学, 大学院医学研究科, 客員研究員 (70507574)
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| Co-Investigator(Renkei-kenkyūsha) |
TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 唇顎口蓋裂 / 再生医療 / G-CSF / 徐放化 / β-TCP |
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| Research Abstract |
A total of 27 calvarial defects were randomly divided into nine treatment groups. One set of defects was filled with a beta-TCP disc containing normal saline alone (group-A, control) or 1 (group-B), 5 (group-C), or 20 ug of G-CSF saline solution (group-D). Another set of defects was filled with a beta-TCP disc with an overlaid gelatin hydrogel sheet incorporating normal saline alone (group-E), or 1 (group-F), 5 (group-G), or 20 ug of G-CSF solution (group-H). The last set of defects was left untreated (group-I). Histologic and histomorphometric analyses were performed at eight weeks postoperatively.
In groups F and G, values of newly formed bone were significantly higher compared to group A. The percentage of remaining beta-TCP in group G was significantly lower compared to group A.
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