2012 Fiscal Year Final Research Report
Investigation of nerve regeneration and its plasticity using a new antibodies against phosphorylated GAP-43 (p-GAP-43)
| Project/Area Number |
23659830
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | p-GAP-43 / end to side coaptation / epineurial window / nerve injury / perineurial window |
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| Research Abstract |
Histological changes were observed in peripheral nerves following end-to-side nerve coaptation to determine the effects of perineurial opening and deliberate donor nerve injury during surgery. Seven days after coaptation of the musculocutaneous (recipient) nerve to the ulnar (donor) nerve, the nerves were immunohistochemically analysed using antibodies against neurofilament-H (RT97) and phosphorylated GAP-43 (p-GAP-43). The latter was found to detect specifically generating nerve fibers. Statistical evaluation revealed increased efficacy of perineurial opening and deliberate donor nerve injury in end-to-side nerve coaptation, suggesting that partial nerve fiber herniation with partial axonotmesis or neurotomesis was important for effective axonal regeneration in end-to-side nerve coaptation.
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