Involvement of claudin-network in the development of ARDS caused by influenza virus infection

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659841
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Tohoku University
• Principal Investigator: KAWAKAMI Kazuyoshi 東北大学, 大学院・医学系研究科, 教授 (10253973)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazuo 千葉大学, 大学院・医学研究院, 特任教授 (20192130)
• Co-Investigator(Renkei-kenkyūsha): AOYAGI Tetsuji 東北大学, 大学院・医学系研究科, 助教 (50581609)
• Project Period (FY): 2011 – 2012
• Keywords: ARDS / インフルエンザ / タイトジャンクション / クローディン

Research Abstract

We addressed the possible involvement of claudins in the development of acute lung injury. In a real-time PCR analysis, expression of cluadin 2 and 4 was increased in the injured lungs, whereas that of claudin 5, 6, 9 and 18-1 was reduced. In an immunohistochemical analysis, expression of claudin 2 and 4 was detected in the bronchoepithelial cells of injured lungs. Lung edema caused by LPS administration tended to be worsened in claudin4 gene-disrupted mice. These results suggest that claudins may be involved in thedevelopment of ARDS.

Research Products

• [Journal Article] Involvement of High Mobility Group Box 1 and the therapeutic effect of recombinant thrombomodulin in a mouse model of severe acute respiratory distress syndrome (2013)
  • Author(s): Kudo D, Toyama M, Aoyagi T, Akahori Y, Yamamoto H, Ishii K, Kanno E, Maruyam R, Kaku M, Kushimoto S, Kawakami K
  • Journal Title: Clin. Exp. Immunol Volume: (In press)
  • DOI: doi:10.1111/cei.12106
  • Peer Reviewed
• [Journal Article] Low-dose interferon-α treatment improves survival and inflammatory responses in a mouse model of fulminant acute respiratory distress syndrome (2013)
  • Author(s): Kudo D, Uno K, Aoyagi T, Akahori A, Ishii K, Kanno E, Maruyama R, Kushimoto S, Kaku M, Kawakami K
  • Journal Title: Inflammation Volume: (In press)
  • DOI: doi:10.1007/s10753-013-9607-1
  • Peer Reviewed
• [Journal Article] Contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection. Microbiol (2012)
  • Author(s): Sugamata R, Dobashi H, Nagao T, Yamamoto K, Nakajima N, Sato Y, Aratani Y, Oshima M, Sata T, Kobayashi K, Kawachi S, Nakayama T, Suzuki K
  • Journal Title: Immunol Volume: 56 Pages: 171-182
  • Peer Reviewed
• [Journal Article] 病態生理,第2章病理・病態生理「新しい診断と治療のABC肺炎改訂第2版」 (2011)
  • Author(s): 工藤大介,久志本成樹,川上和義
  • Journal Title: 最新医学別冊,最新医学社 Pages: 39-45
• [Presentation] マウス劇症型ARDSモデルに対するrTMの治療効果とその作用機序 (2013)
  • Author(s): 工藤大介,外山真彦,石井恵子,久志本成樹,川上和義
  • Organizer: 第35回日本血栓止血学会学術集会
  • Place of Presentation: 山形
  • Year and Date: 20130531-0601
• [Presentation] Contributions of High Mobility Group Box 1 in experimental fulminant acute respiratory distress Syndrome (2012)
  • Author(s): Kudo D, Ishii K, Kushimoto S, Kawakami K
  • Organizer: 7th Congress of the International Federation of Shock Societies and 35th Annual Conference on Shock
  • Place of Presentation: Miami Beach, FL, USA
  • Year and Date: 20120609-13
• [Presentation] Anti-inflammatory and therapeutic effects of interferon-α in a mouse model of acute respiratory distress syndrome (2011)
  • Author(s): Kudo D, Uno K, Ishii K, Kushimoto S, Kawakami K
  • Organizer: 第40回日本免疫学会学 術集会
  • Place of Presentation: 千葉
  • Year and Date: 20111127-29