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2011 Fiscal Year Final Research Report

Expression of WT1 natural ant-sense NA and osteoclastogenesis : A novel strategy for the regulation of bone destruction

Research Project

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Project/Area Number 23659860
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

KUKITA Toshio  九州大学, 歯学研究院, 教授 (70150464)

Co-Investigator(Kenkyū-buntansha) KUKITA Akiko   (30153266)
Project Period (FY) 2011
Keywords口腔解剖学(含組織学・発生学)
Research Abstract

WT1, found as the gene product of infant kidney tumor(Wilms' tumor), is azinc-finger transcription factor and known to have ability to induce macrophagedifferentiation, however, little is known on the involvement of WT1 gene expression inosteoclastogenesis. RNA in situ hybridization studies using mandibule of new bornrats revealed that osteoclasts expressed abundant WT1 anti-sense RNAs. Northernblotting analysis and anti-sense-specific rtPCR confirmed the actual expression of WT1anti-sense RNA in mandibule of new born rats. Time course studies concerning theexpression of WT1 protein in in vitro osteoclastogenesis using RAW-D cells showedthat WT1 expression observed at undifferentiated stages markedely suppressed if cellswere stimuated to differentiate to osteoclasts. When endogeneous WT1 expression wasknocked down by transfecting anti-sense WT1 expressing vector into RAW-cells, marked stimulation of osteoclastogenesis was observed. These results strongly suggestthat WT1 protein has a regulatory role in the regulation of osteoclast differentiation. This research also revealed a possible involvement of WT1 anti-sense RNA in theregulation of osteoclastogenesis.

  • Research Products

    (12 results)

All 2012 2011 Other

All Journal Article (3 results) Presentation (9 results)

  • [Journal Article] The Transcription Factor FBI-1/OCZF/LRF Is Expressed in Osteoclasts and Regulates RANKL-Induced Osteoclast Formation In Vitro and In Vivo2011

    • Author(s)
      KukitaA., Kukita T., Nagata K., Teramachi J., Li Y-J., Yoshida H., Miyamoto H., Gay S., Pessler F., Shobuike T.
    • Journal Title

      Arth Rheumatism

      Volume: 63(9) Pages: 2744-2754

  • [Journal Article] Adenosine abolishes MTX-induced suppression of osteoclastogenesis and inflammatory bone destruction in adjuvant-induced arthritis2011

    • Author(s)
      Teramachi J., Kukita A., Li Y-J., Ushijima Y., OhkumaY., Wada N., Watanabe T., Nakamura S., Kukita T.
    • Journal Title

      Lab. Invest

      Volume: 91 Pages: 719-731

  • [Journal Article] Infection of RANKL-primed RAW-D macrophages with Porphyromonas gingivalis promotes osteoclastogenesis in a TNF-α-independent manner

    • Author(s)
      Kukita A., Ichigi Y., Takigawa I., Watanabe T., Kukita T., Miyamoto H
    • Journal Title

      PLoS ONE

      Volume: in press

  • [Presentation] WT1アンチセンスRNAの成熟破骨細胞に於ける高発現と分化制御機能2012

    • Author(s)
      李銀姫、久木田明子、久木田敏夫
    • Organizer
      第117回日本解剖学会
    • Place of Presentation
      山梨
    • Year and Date
      20120300
  • [Presentation] 間葉系幹細胞による炎症性骨破壊制御2011

    • Author(s)
      高野登志夫、李銀姫、久木田明子、山座孝義、高橋明、鮎川保則、古谷野潔、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Year and Date
      20110900
  • [Presentation] 膜ナノチューブによる前破骨細胞融合制御2011

    • Author(s)
      高橋良、久木田明子、李銀姫、鮎川保則、古谷野潔、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Year and Date
      20110900
  • [Presentation] ヒト過剰歯由来幹細胞の免疫細胞療法効果について2011

    • Author(s)
      牧野友祐、山座孝義、山座治義、馬蘭、益田啓太郎、園田総一朗、城戸瑞穂、野中和明、寺田善博、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Year and Date
      20110900
  • [Presentation] 乳歯凍結保存法がヒト乳歯由来幹細胞の免疫調節能に与える影響について2011

    • Author(s)
      山座孝義、牧野友祐、山座治義、馬蘭、園田総一朗、益田啓太郎、野中和明、寺田善博、久木田敏夫
    • Organizer
      第53回歯科基礎医学会
    • Place of Presentation
      岐阜
    • Year and Date
      20110900
  • [Presentation] Mesenchyme stem cells markedly suppressed inflammatory bone destruction in rats with adjuvant-induced arthritis.2011

    • Author(s)
      Toshio Takano, Yin-ji Li, Akiko Kukita, Takayoshi Yamaza, Yasunori Ayukawa, Kiyoshi Koyano, Toshio Kukita
    • Organizer
      American Society for Bone and Mineral Research(ASBMR) Annual Meeting
    • Place of Presentation
      SanDiego, USA
    • Year and Date
      20110900
  • [Presentation] Involement and regulatory role of membrane nanotubes in fusion of osteoclast precursors : A possible migration and penetration of DC-STAMP protein into osteoclast precursors through intercellular bridges2011

    • Author(s)
      Akira Takahashi, Akiko Kukita, Yin-ji Li, Hisayuki Nomiyama, Yasunori Ayukawa, Kiyoshi Koyano, Toshio Kukita
    • Organizer
      American Society for Bone and Mineral Research(ASBMR) Annual Meeting
    • Place of Presentation
      SanDiego, USA
    • Year and Date
      20110900
  • [Presentation] Nordihydroguaiaretic Acidは破骨細胞分化とラットアジュバント関節炎における骨破壊を抑制する2011

    • Author(s)
      李銀姫、久木田明子、屈鵬飛、高野登志夫,實松敬介、二ノ宮祐三、久木田敏夫
    • Organizer
      第29回日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      20110700
  • [Presentation] 間葉系幹細胞による骨破壊制御:アジュバント関節炎ラットを用いた解析2011

    • Author(s)
      高野登志夫、李銀姫、久木田明子、山座孝義、鮎川保典小谷野潔、久木田敏夫
    • Organizer
      第29回日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      20110700

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Published: 2013-07-31  

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