2012 Fiscal Year Final Research Report
Trial research of fibrous dysplasia model transplanted with GNAS1 mutant cells
| Project/Area Number |
23659877
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Osaka University |
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Principal Investigator |
TOYOSAWA Satoru 大阪大学, 大学院・歯学研究科, 教授 (30243249)
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| Co-Investigator(Kenkyū-buntansha) |
SAEKI Makio 大阪大学, 大学院・歯学研究科, 講師 (30273692)
SATO Jun 大阪大学, 大学院・歯学研究科, 講師 (70335660)
USAMI Yu 大阪大学, 歯学部附属病院, 助教 (80444579)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 線維性骨異形成症 / GNAS1遺伝子 / 骨髄間質細胞 / 遺伝子診断 |
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| Research Abstract |
To replacement Arg201 with His in the Gs, we produced mutated GNAS1 gene (R201H) replacing with A instead of G of the human GNAS1 gene, which causes human fibrous dysplasia of bone (FD). To stably transfer the R201H into cultured cells, we constructed a lentiviral vector expressing R201H, and used it to transduce C57BL/6 mice-derived marrow stromal cells. We are now elucidating the cell proliferation and differentiation of the R201H-expressing cells and are going to make a mouse model by the orthotopic transplant of the transduced cells. In addition, we studied the method of highly precise genetic tests that we could distinguish it from other similar bone lesions. As a result, the real-time PCR using PNA probe and pyrosequencing method were more effective, compared to conventional PCR-restriction fragment length polymorphism.
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[Journal Article] Loss of B-catenin induces multifocal periosteal chondroma-like masses in mice.2013
Author(s)
Cantley L, Saunders C, Guttenberg M, Candela ME, Ohta Y, Yasuhara R, Kondo N, Sgariglia F, Asai S, Zhang X, Qin L, Hecht JT, Chen D, Yamamoto M, Toyosawa S, Dormans JP, Esko JD, Yamaguchi Y, Iwamoto M, Pacifici M, Enomoto-Iwamoto M
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Journal Title
Am J Pathol
Volume: 182
Pages: 917-27
Peer Reviewed
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