2013 Fiscal Year Final Research Report
Stem cell culture system with optimal spaces and anti-gravitational stimuli
| Project/Area Number |
23659907
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | ３次元培養 / アクチン / コラーゲン / 骨原細胞 / 反重力装置 / チタン不織布 / チタン結合蛋白 / 反重力効果 |
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| Research Abstract |
We report here a new invention of an anti-gravity device for cell culture, in that we can invert the position of conventional culture dishes up-side-down in the culture medium. Also, we can rotate the 3D scaffolds of any kind in culture medium by this device. Our hypothesis is: if we change the direction of gravitational force on cell systematically, we can eventually detect the effects of gravity on the behaviors of cells. In this paper we described the details of the structures and concept of anti-gravitational device, and also some of the preliminary results of application of the device for osteoblasts MC-3T3 E1 and cancer cells MG 63. Results included remarkable morphological changes of the cells under the anti-gravitational environment, with 10-20 times higher RNA values of actin and collagen, indicating total rearrangement of cyto-skeletons and protein productions.
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[Journal Article] Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold2014
Author(s)
Yoshinori Kuboki, Toshitake Furusawa, Masaaki Sato, Yongkun Sun, Hidero Unuma, Shigeaki Abe, Ryuichi Fujisawa, Tsukasa Akasaka, Fumio Watari, Hiroko Takita, Rachel Sammons
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Journal Title
Biomedical Materials and Engineering
Volume: Vol.24, Number3
Pages: 1539-1548
DOI
Peer Reviewed
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[Journal Article] Interaction between titanium and phosphoproteins revealed by chromatography column packed with titanium beads2012
Author(s)
Yoshinori Kuboki, Toshitake Furusawa, Masaaki Sato, Sun Yongkun, Hidero Unuma, Ryuichi Fujisawa, Shigeaki Abe, Tsukasa Akasaka, Fumio Watari, Hiroko Takita, Rachel Sammons
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Journal Title
Bio-Medical Materials and Engineering
Volume: Vol.22, No.5
Pages: 283-288
DOI
Peer Reviewed
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[Presentation] チタンとタンパク質の反応についてー その基礎と応用2013
Author(s)
久保木 芳徳,古沢 利武, 佐藤 正明, 孫 永琨,鵜沼 英郎,滝田 裕子, 中沖 靖子, 藤澤 隆一, 阿部 薫明, 赤坂 司, 亘理 文夫, 小川 真吾, R. L. Sammons
Organizer
生物物理学会北海道支部会
Place of Presentation
北海道大学・理学部(札幌市)
Year and Date
2013-03-05
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[Presentation] チタン製の人工骨・人工歯根が骨に結合する生化学的メカニズム2013
Author(s)
久保木 芳徳,古沢 利武, 佐藤 正明, 孫 永琨,鵜沼 英郎,滝田 裕子,藤澤 隆一, 阿部 薫明, 赤坂 司, 亘理 文夫, Rachel L. Sammons
Organizer
第25回代用臓器・再生医学研究会
Place of Presentation
社会医療法人北楡会 札幌北楡病院内(札幌市)
Year and Date
2013-02-23
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[Presentation] Sammon Biochemical Mechanism of Binding between Titanium Implant and Bone : Interaction of Phosphoproteins on the Surface of Titanium2012
Author(s)
Yoshinori Kuboki, Toshitake Furusawa, Masaaki Satou, Sun Yongkun, Hiderou Unuma, Ryuichi Fujisawa, Shigeaki Abe, Tsukasa Akasaka, Fumio Watari, Hiroko Takaita and Rachel L
Organizer
9^<th> World Biomaterials Congress
Place of Presentation
Pride International Convention Centre, Chengdu, China
Year and Date
2012-06-02
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[Book] Impact of carbon nanotubes upon protein chemistry. In Navam S. Hettiarachchy (Ed), Food Protein and Peptides (Chemistry, Functionality, Interactions, and Commercialization)2012
Author(s)
Kuboki, Y., Fujisawa, R., Watari, F. and Sammons, L. R
Total Pages
454 (423-429)
Publisher
CRC Press, Taylor & Francis Group, Boca Raton, London, New York
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