2012 Fiscal Year Final Research Report
Complexity single cell analysis designed to create a new periodontal regenerative medicine in silico
| Project/Area Number |
23659917
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Shinya 大阪大学, 大学院・歯学研究科, 教授 (70239490)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Satoru 大阪大学, 歯学部附属病院, 講師 (40359849)
TAKEDACHI Masahide 大阪大学, 大学院・歯学研究科, 助教 (60452447)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 歯周組織再生 / 歯根膜組織 / 単細胞イメージング / 複雑系単細胞研究 |
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| Research Abstract |
Real-time live cell imaging revealed that FGF-2 promoted the cell cycle and the division of the cells. Furthermore, FGF-2 stimulated migration of periodontal ligament cells in a would healing model. In this model, VEGF further enhanced the FGF-2-induced migration. It was also demonstrated that co-culture of endothelial cells with periodontal ligament cells stimulated the lumen formation of the endothelial cells and this effect was partially caused by VEGF produced by FGF-2-stimulated periodontal ligament cells. These results revealed, at least in part, the mechanisms of periodontal regeneration by FGF-2.
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