Development of micro-fluid-path type bio -chips using magnetic micro-beads for dental tissue re -generation medicine

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659923
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: TAIRA Masayuki 岩手医科大学, 歯学部, 准教授 (60179398)
• Co-Investigator(Renkei-kenkyūsha): NEZU Takashi 岩手医科大学, 歯学部, 講師 (40264056) ; SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133)
• Project Period (FY): 2011 – 2012
• Keywords: 歯科医用工学 / マイクロ流路 / 微小ポンプ / 2液混合 / 幹細胞 / 細胞分画 / 混合チップ / 微小ビーズ

Research Abstract

We tried to produce trial micro-fluid-path type bio -chips using Micro Electro Mechanical Systems (MEMS) technology and magnetic micro-beads under development. We examined biding efficiencies between target cells labeled with biotin-conjugated antibodies a nd streptavidin-bound magnetic beads. We obtained partial success in collecting target cells after passing solutions containing antibody -biotin-labeled cells and streptavidin-bound magnetic mico -beads through micro-fluid-paths in trial bio-chips.

Research Products

• [Journal Article] Subcutaneous tissue reactions against nano-apatite collagen composites (2012)
  • Author(s): Hatakeyama, W., Taira, M. , Kihara, H., Sasaki, M., Kimura, S. and Kondo, H.
  • Journal Title: ano Biomedicine Volume: 4(2) Pages: 118-124
  • Peer Reviewed
• [Journal Article] Quantitative analyses of osteogenic-differentiation -related gene expressions in human osteoblasts-like cells (SaOS-2) cultured on hydroxyapatite and titanium. J (2012)
  • Author(s): Taira, M., Hatakeyama, W., Kihara, H., Kondo, H., Ueda, K. and Narushima T.
  • Journal Title: Oral Tissue Eng Volume: 10(1) Pages: 34-41
  • Peer Reviewed
• [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage-recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC50 nickel ions (2011)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M. and Kimura, S
  • Journal Title: Nano-Biome Volume: 3(2) Pages: 294-299
  • URL: http://www.nanobio.jp/n3-2-7e.html http://www.nanobio.jp/n4-2-7e.html
  • Peer Reviewed
• [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper (2+) ions (2011)
  • Author(s): Taira M, , Shimoyama Y ., Kagiya T ., Sasaki M., Nezu T., Harada H ., Kimura S.
  • Journal Title: Dent Mater J Volume: 30(3) Pages: 293-299
  • URL: http://dx.doi.org/10.4012/dmj.2010-088
  • Peer Reviewed
• [Presentation] マイクロ流路チップを用いた細胞と磁性ビーズ溶液の混合に関する研究 (2013)
  • Author(s): 平 雅之,畠山 航,鬼原 英道,近藤 尚知
  • Organizer: 第61回日本歯科理工学会学術講演会
  • Place of Presentation: 東京都
  • Year and Date: 2013-04-13
• [Presentation] 口腔バイオフィルム構成多糖の凝集状態のQCMによる解析 (2012)
  • Author(s): 根津尚史,佐々木かおり,齋藤設雄,平雅之
  • Organizer: 第60回日本歯科理工学会学術講演会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-10-13
• [Presentation] 多孔質アパタイト粒子コラーゲン複合体によるラット頭蓋骨クリティカルサイズ骨欠損部における骨再生の試み (2012)
  • Author(s): 畠山 航,平 雅之,鬼原 英道,近藤 尚知
  • Organizer: 第60回日本歯科理工学会学術講演会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-10-13
• [Presentation] ナノアパタイト(nano -SHAp)の材料学的評価並びに生体反応評価 (2012)
  • Author(s): 畠山 航,平 雅之,鬼原 英道,近藤 尚知
  • Organizer: 第59回日本歯科理工学会学術講演会
  • Place of Presentation: 徳島市
  • Year and Date: 2012-04-14
• [Presentation] THP -1細胞を用いたチタン微粒子、銅イオンとTEGDMAモノマーの細胞障害性の評価 (2011)
  • Author(s): 平 雅之
  • Organizer: 日本動物実験代替法学会 第24回大会
  • Place of Presentation: Sendai
  • Year and Date: 2011-11-12
• [Presentation] バイオフィルム関連高分子マトリックス中の薬剤透過性の定量評価の試み (2011)
  • Author(s): 根津尚史,佐々木かおり,齋藤設雄
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 郡山
  • Year and Date: 2011-10-23
• [Presentation] チタンへの金蒸着がチオール化合物の付着に及ぼす影響 (2011)
  • Author(s): 齋藤設雄,佐々木かおり,根津尚史,平雅之
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 郡山
  • Year and Date: 2011-10-23
• [Presentation] TEGDMAに曝された時のTHP -1単球の異物代謝並びに炎症性サイトカイン関連遺伝子の発現評価 (2011)
  • Author(s): 平 雅之
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 郡山
  • Year and Date: 2011-10-22
• [Presentation] 剤徐放性コラーゲン基材の調製-架橋法による架橋効率の比較- (2011)
  • Author(s): 佐々木かおり,齋藤設雄,根津尚史,平雅之
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 郡山
  • Year and Date: 2011-10-22
• [Presentation] マクロファージが銅イオンに暴露された時のmicroRNAの発現について (2011)
  • Author(s): 鍵谷忠慶,平 雅之,安藤禎紀,藤村朗
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-02
• [Presentation] Histological and TEM observation of subcutaneous tissues exposed to particulate pure metals (2011)
  • Author(s): Saitoh, S., Sasaki, K., Nezu, T. and Taira, M.
  • Organizer: International Dental Materials Congress 2011 (IDMC2011)
  • Place of Presentation: Soul, Korea
  • Year and Date: 2011-05-28
• [Presentation] Diffusion of an antimicrobial acriflavine through a concentrated solution of hyaluronic acid as a matrix component of biofilms (2011)
  • Author(s): Nezu, T., Sasaki, K., Saitoh, S. and Taira, M.
  • Organizer: International Dental Materials Congress 2011 (IDMC2011)
  • Place of Presentation: Soul, Korea
  • Year and Date: 2011-05-28
• [Remarks]
  • URL: http://hitech-d.iwate-med.ac.jp/dmst/index-j.htm