2013 Fiscal Year Final Research Report
The analysis of LPS aggregates derived from aqueous phase of two-phase separation system
| Project/Area Number |
23659985
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
FUJISE Osamu 九州大学, 大学病院, 講師 (40315099)
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| Co-Investigator(Renkei-kenkyūsha) |
MIURA Mayumi 九州大学, 歯学研究院, 助教 (00404054)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 二相分配法 / リポ多糖 / 歯周病原細菌 / 高次構造 / DNA |
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| Research Abstract |
In our previous study, it was observed that LPS from periodontopathic Aggregatibacter actinomycetemcomitanswas detected in aqueous phase, in addition to detergent phase, after two-phase separation system. The aim of this study was to examine how LPS remained in aqueous phase. Our results suggest that LPS in aqueous environment forms aggregations via ionic bridge involving divalent cations, beside hydrophobic association. This ionic force in LPS aggregates might promote resistance against detergent. In addition, this study established the method to purify DNA-LPS complex located cell surface, and the complex formed via non-ionic association was found to be detected in aqueous phase of two-phase separation system. It should be examined if the non-ionic DNA-LPS complex has different virulence from the ionic LPS aggregate.
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