2012 Fiscal Year Final Research Report
Dendritic coding of sensory and motor information
| Project/Area Number |
23680043
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fusional basic brain science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MURAYAMA Masanori 独立行政法人理化学研究所, 行動神経生理学研究チーム, チームリーダー (30578901)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 新皮質 / 体性感覚 / 知覚 / 樹状突起 / カルシウムイメージング |
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| Research Abstract |
We aimed to understand how L5 neuron dendrites work in vivo. For this purpose, we developed a fast 3-dimentional scanning 2-photon microscope to achieve dendritic Ca^<2+> imaging with optogenetical manipulation, and local drug applications to a brain area. Dendritic Ca^<2+> activity could be evoked by hindlimb stimulations and electrical stimulations to a higher-order motor cortex in anesthetized mice. This dendritic Ca^<2+> activity significantly increased during awake state. We also found that enlarged dendritic activity significantly suppressed by local application of TTX, a antagonist of sodium channel, to the higher-order motor area. These results indicate that large dendritic activity in awake state can be activated by the higher-order motor area.
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