2013 Fiscal Year Final Research Report
Development of Integrated Transistor Based device for Monitoring of Enzymatic Activities as Next-generation Platform for Apoptosis Pathways Analysis
| Project/Area Number |
23680051
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MATSUMOTO Akira 東京医科歯科大学, 生体材料工学研究所, 准教授 (70436541)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 電界効果トランジスタ / アポトーシス / カスパーゼ / 酵素活性 / パスウェイ解析 |
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| Research Abstract |
We envisioned the development of an integrated transistor based device that is able to quantify enzymatic activities in cells. Caspase-3 and -8 were studied as targets relevant to apoptosis pathway analysis. A series of peptides containing each Caspase-specific amino acid sequence along with spacer and cysteine moiety were prepared so that they will have good enzyme-accssibility and affinity to gold for the immobilization. Mass-spectroscopic analysis uncovered that these designed reactions take place in quantitative and each substrate-specific fashion. This reaction could also be visualized on the basis of the coloration of gold nano-particle. Furthermore, it was compatible with potentiometric detection format, indicating the validity of our approach.
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