Innovative multi-molecular imaging strategy toward comprehensive understanding of food factor signaling response contributed to the health promotion

2013 Fiscal Year Final Research Report

• Project/Area Number: 23680073
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyushu University
• Principal Investigator: FUJIMURA Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 質量分析イメージング / 食シグナル / メタボローム / プロテオーム / MALDI-MS / マルチオミクスイメージング

Research Abstract

In this study, we attempted to understand the potential comprehensive relationship among a series of endogenous molecules with spatiotemporal information within tissue micro-regions for elucidating specific biological response to an orally dosed functional food factor. We succeeded in developing new techniques capable of two-dimensionally visualizing metabolome and proteome within tissue micro-regons, namely, multi-omics imaging, by improving a MALDI-MS technique. In addition, this analytical approach enabled us to simultaneously visualize orally dosed green tea catechin and metabolome within tissue micro-regions, and further visualized co-localization of green tea catechin and its target molecule. These results will provide a novel basic technology for understanding of precise food factor signaling response.

Research Products

• [Journal Article] MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals (2014)
  • Author(s): Fujimura Y and Miura D
  • Journal Title: Metabolites Volume: 4 Pages: 319-346
  • DOI: 10.3390/metabo4020319
  • Peer Reviewed
• [Journal Article] In situ label-free imaging for visualizing the biotransformation of a bioactive polyphenol (2013)
  • Author(s): Kim YH, Fujimura Y, Hagihara T, Sasaki M, Yukihira D, Nagao T, Miura D, Yamaguchi S, Saito K, Tanaka H, Wariishi H, Yamada K, Tachibana H
  • Journal Title: Sci. Rep. Volume: 3 Pages: 2805
  • DOI: 10.1038/srep02805
  • Peer Reviewed
• [Journal Article] Tannic acid, a higher galloylated pentagalloylglucose, suppresses antigen-specific IgE production by inhibitingɛgermline transcription induced by STAT6 activation (2013)
  • Author(s): Kim YH, Yoshimoto M, Nakayama K, Tanino S, Fujimura Y, Yamada K, Tachibana H
  • Journal Title: FEBS Open Bio. Volume: 3 Pages: 341-345
  • DOI: 10.1016/j.fob.2013.07.008
  • Peer Reviewed
• [Journal Article] Green tea polyphenol EGCG sensing motif on the 67-kDa laminin receptor (2012)
  • Author(s): Fujimura Y, Sumida M, Sugihara K, Tsukamoto S, Yamada K, Tachibana H
  • Journal Title: PLoS One Volume: 7 Pages: e37942
  • DOI: 10.1371/journal.pone.0037942
  • Peer Reviewed
• [Journal Article] In situ metabolomic mass spectrometry imaging : recent advances and difficulties (2012)
  • Author(s): Miura D, Fujimura Y, Wariishi H
  • Journal Title: J . Proteomics Volume: 75 Pages: 5052-5060
  • DOI: 10.1016/j.jprot.2012.02.011
  • Peer Reviewed
• [Journal Article] Metabolomics-driven nutraceutical evaluation of diverse green tea cultivars (2011)
  • Author(s): Fujimura Y, Kurihara K, Ida M, Kosaka R, Miura D, Wariishi H, Maeda-Yamamoto M, Nesumi A, Saito T, Kanda T, Yamada K, Tachibana H
  • Journal Title: PLoS One Volume: 6 Pages: e23426
  • DOI: 10.1371/journal.pone.0023426
  • Peer Reviewed
• [Presentation] 質量分析イメージングによる傷害ストレス条件下でのトマト果実代謝物分布の可視化 (2014)
  • Author(s): 中村純也,三浦大典,高橋勝利,藤村由紀,割石博之
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 川崎市
  • Year and Date: 20140327-30
• [Presentation] Time-Dependent Spatiotemporal Metabolic Variance in Mouse Kidney upon Cisplatin-Induced Acute Renal Injury (2014)
  • Author(s): Irie M, Honda Y, Fujimura Y, Setoyama D, Hyodo F, Yamato M, Miura D, Wariishi H
  • Organizer: SF RRI2014
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20140323-26
• [Presentation] 緑茶の機能性を捉える低分子ケミカルセンシングに関する研究 (2014)
  • Author(s): 藤村由紀
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京都
  • Year and Date: 2014-03-27
  • Invited
• [Presentation] The biological function of tea via EGCG receptor (2014)
  • Author(s): Fujimura Y
  • Organizer: The 2014 Annual Meeting and Symposium of Health F ood Science of Taiwan
  • Place of Presentation: Keelung, Taiwan (Invited)
  • Year and Date: 2014-03-07
• [Presentation] In situ label-free visualization of the phase II metabolism of a green tea polyphenol (2013)
  • Author(s): Kim YH, Fujimura Y, Sasaki M, Yukihira D, Miura D, Saito K, Wariishi H, Yamada K, and Tachibana H
  • Organizer: ICOS2013
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 20131106-08
• [Presentation] 緑茶成分ストリクチニンのin situ質量分析イメージング法の開発 (2013)
  • Author(s): 佐々木雅子,金允喜,藤村由紀,行平大地,松本結実,三浦大典,割石博之,山田耕路,立花宏文
  • Organizer: 第8回メタボロームシンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 20131003-04
• [Presentation] 低分子食品成分の組織内微小空間分布の非標識可視化法 (2013)
  • Author(s): 藤村由紀,三浦大典,割石博之,立花宏文
  • Organizer: 第67回日本栄養・食糧学会大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2013-05-25
  • Invited
• [Presentation] 組織内微小領域における機能性食品因子の時空間分解可視化 (2013)
  • Author(s): 藤村由紀,三浦大典,割石博之,立花宏文
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台市
  • Year and Date: 2013-03-27
  • Invited
• [Presentation] Identification of green tea polyphenol EGCG sensing motif on 67-kDa laminin receptor (2012)
  • Author(s): Sumida M, Fujimura Y, Sugihara K, Tsukamoto S, Yamada K, and Tachibana H
  • Organizer: The 25th Annual and International Meeting of J apanese Association for Animal Cell Technology
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20121127-30
• [Presentation] 緑茶カテキンの組織内二次元分布情報を非標識可視化する質量分析イメージング法の開発 (2012)
  • Author(s): 金允喜,藤村由紀,萩原立春,行平大地,山口歩,永尾達彦,齋藤和徳,三浦大典,割石博之,山田耕路,立花宏文
  • Organizer: 第17回日本フードファクター学会学術集会および第9回日本カテキン学会合同大会
  • Place of Presentation: 静岡市
  • Year and Date: 20121110-11
• [Presentation] MALDI-MSによる代謝物/タンパク質統合イメージング技術の開発 (2012)
  • Author(s): 山口歩,三浦大典,藤村由紀,割石博之
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 20121023-26
• [Presentation] Development of mass spectrometry imaging technique towards two-dimensional visualization of green tea polyphenol in tissue micro-regions (2012)
  • Author(s): Fujimura Y, Hagihara T, Kim YH, Yukihira D, Yamaguchi A, Miura D, Wariishi H, Yamada K, and Tachibana H
  • Organizer: 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20120520-24
• [Presentation] 食品機能性評価に向けたニュートリメタボロミクス (2012)
  • Author(s): 藤村由紀,三浦大典,割石博之,立花宏文
  • Organizer: 第7回メタボロームシンポジウム
  • Place of Presentation: 鶴岡市
  • Year and Date: 2012-10-11
  • Invited
• [Presentation] 質量分析を基盤としたプロテオミクス技術のメタボローム研究への応用 (2012)
  • Author(s): 藤村由紀
  • Organizer: 第36回蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Place of Presentation: 宮崎市
  • Year and Date: 2012-09-08
  • Invited
• [Presentation] レドックス関連疾患の理解とニュートリメタボロミクス (2012)
  • Author(s): 藤村由紀,三浦大典,大和真由実,兵藤文紀,安川圭司,市川和洋,内海英雄,割石博之,立花宏文
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島市
  • Year and Date: 2012-06-07
  • Invited
• [Presentation] MALDI-MSによるmulti-omicsイメージング技術の開発 (2011)
  • Author(s): 山口歩,三浦大典,藤村由紀,割石博之
  • Organizer: 第6回メタボロームシンポジウム
  • Place of Presentation: 吹田市
  • Year and Date: 20111013-14
• [Presentation] An Integrated In Situ Proteomic and Metabolomic MALDI-MS Imaging toward Multi-Omics Studies (2011)
  • Author(s): Yamaguchi A, Fujimura Y, Miura D, and Wariishi H
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, Colorado
  • Year and Date: 20110605-09
• [Presentation] 緑茶ポリフェノールEGCGの抗腫瘍効果に対する酸素応答性の理解に向けた細胞内代謝物プロファイリング (2011)
  • Author(s): 藤村由紀,塚本俊太郎,入江美穂,三浦大典,割石博之,立花宏文
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20110513-15
• [Presentation] ニュートリメタボロミクスを切り拓く多元的質量分析システムの開発 (2011)
  • Author(s): 藤村由紀
  • Organizer: 日本農芸化学会西日本支部若手シンポジウム
  • Place of Presentation: 宮崎市
  • Year and Date: 2011-09-18
  • Invited
• [Presentation] 食品機能性評価に向けた代謝物プロファイリング技術の開発 (2011)
  • Author(s): 藤村由紀
  • Organizer: 産学官連携技術シーズセミナーin福岡
  • Place of Presentation: 福岡市
  • Year and Date: 2011-09-01
  • Invited
• [Remarks]
  • URL: http://redoxnavi.kyushu-u.ac.jp/group2/