2013 Fiscal Year Final Research Report
Identification of critical microRNAs in gastric cancer and application for epigenetic therapy.
| Project/Area Number |
23680090
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical oncology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | エピジェネティクス / エピジェネティック治療 / マイクロRNA / 胃がん |
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| Research Abstract |
The aim of this study is to investigate the role of microRNAs (miRNAs) during gastric carcinogenesis and the feasibility of epigenetic therapy for gastric neoplasms.MiRNA microarray analyses revealed that miR-29c was significantly down-regulated in gastric cancers and that miR-142 and miR-155 were overexpressed in gastric MALT lymphomas. MiR-1246, miR-302a and miR-4448 were up-regulated by treatment of gastric cancer cells with EZH2 inhibitors such as SAHA and DZNep. In addition, we have developed a novel miRNA promoter microarray for chromatin immunoprecipitation (ChIP)-on-chip assay. Using this custom-made miRNA promoter microarray, we have successfully performed ChIP-on-chip assay to identify miRNAs regulated by histone modification. These results indicate that misexpression of miRNAs plays critical roles during gastric carcinogenesis and that epigenetic therapy with chromatin-modifying drugs exert multiple anti-cancer effects through activation of tumor-suppressor miRNAs.
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[Journal Article] Bile acids increase levels of microRNAs 221 and 222, leading to degradation of CDX2 during esophageal carcinogenesis2013
Author(s)
Matsuzaki J, Suzuki H, Tsugawa H, Watanabe M, Hossain S, Arai E, Saito Y, Sekine S, Akaike T, Kanai Y, Mukaisho KI, Auwerx J, Hibi T
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Journal Title
Gastroenterology
Volume: 145(6)
Pages: 1300-11
DOI
Peer Reviewed
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[Journal Article] Suppressive Effect of the Histone Deacetylase Inhibitor, Suberoylanilide Hydroxamic Acid (SAHA), on Hepatitis C Virus Replication via Epigenetic Changes in Host Cells2013
Author(s)
Sato A, Saito Y, Sugiyama K, Sakasegawa N, Muramatsu T, Fukuda S, Yoneya M, Kimura M, Ebinuma H, Hibi T, Saito H
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Journal Title
J Cell Biochem
Volume: 114 (9)
Pages: 1987-96
DOI
Peer Reviewed
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[Journal Article] Overexpression of miR-142-5p and miR-155 in gastric mucosa-associated lymphoid tissue (MALT) lymphoma resistant to Helicobacter pylori eradication2012
Author(s)
Saito Y, Suzuki H, Tsugawa H, Imaeda H, Matsuzaki J, Hirata K, Hosoe N, Nakamura M, Mukai M, Saito H, Hibi T
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Journal Title
PLoS One
Volume: 7(11)
Pages: e47396
DOI
Peer Reviewed
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[Presentation] The tumor suppressor microRNA-122 is suppressed in liver cancer cells and can be reactivated by DNA methylation inhibitors2013
Author(s)
Matsuura M, Saito Y, Doke Y, Yoshida T, Genka A, Muramatsu T, Kimura M, Arai E, Kanai Y, Saito H
Organizer
Digestive Disease Week 2013
Place of Presentation
Orlando, FL.
Year and Date
20130500
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