in vitro artificial extracellular microenvironments to investigate the mechanisms of cell regulations

2012 Fiscal Year Final Research Report

• Project/Area Number: 23681028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Kyoto University
• Principal Investigator: KAMEI Ken-ichiro 京都大学, 物質-細胞統合システム拠点, 特定拠点助教 (00588262)
• Project Period (FY): 2011 – 2012
• Keywords: マイクロバイオシステム / ヒト ES/iPS 細胞 / 細胞外微小環境

Research Abstract

Cells in a body or tissues are placed in cellular microenvironments, and regulated their functions as well as fate decision in a highly regulated manner. In this study, I tried to create artificial cellular microenvironments with microfluidic and nanotechnologies to establish a new regulatory system for human pluripotent stem cells (hPSCs). The obtained results and findings will contribute the advancement of regenerative medicine and drug development using hPSCs.

Research Products

• [Journal Article] Phenotypic and transcriptional modulation of human pluripotent stem cells induced by nano/microfabrication materials (2013)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, Q. Yuan, M. Nakajima, Y. Chen, and O. Tabata
  • Journal Title: Advanced Healthcare Materials Volume: 2(2) Pages: 287-291
  • DOI: DOI:10.1002/adhm.201200283
• [Journal Article] Fabrication of gelatin nanopatterns for cell culture studies (2013)
  • Author(s): S. S. Li, J. Shi, L. Liu, J. J. Li, L. M. Jiang, C. X. Luo, K. Kamei and Y. Chen
  • Journal Title: Microelectron. Eng. Volume: (in press)
  • DOI: DOI:10.1016/j.mee.2013.01.053
• [Journal Article] Cutting-edge microfabricated biomedical tools for human pluripotent stem cell research
  • Author(s): K. Kamei
  • Journal Title: Journal of Laboratory Automation Volume: (in press)
• [Journal Article] Integration of porous coordination polymers and gold nanorods into core-shell mesoscopic composites towards light-induced molecular release
  • Author(s): K. Khaletskaya, J. Reboul, M. Meilikhov, M. Nakahama, S. Diring, S. Isoda, F. Kim, K. Kamei, R. A. Fischer, S. Kitagawa and S. Furukawa
  • Journal Title: Journal of American Chemical Society Volume: (Published on line)
  • DOI: DOI:10.1021/ja403108x
• [Presentation] Phenotypic modulation of pluripotent stem cells (PSCs) induced by microfabrication materials. (2012)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, M. Nakajima, Q. Yuan, Y. Chen and O. Tabata
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20121028-1102
• [Presentation] 錯体フレームワークを用いた光駆動型生体ガス放出材料 (2012)
  • Author(s): 金 致源・Diring, Stephane・亀井 謙一郎・北川 進・古川 修平
  • Organizer: 日本化学会第92 春季年会
  • Year and Date: 20120325-28
• [Presentation] 錯体フレームワークと生体適合材料の融合による機能性細胞外マトリクスの構 築 (2012)
  • Author(s): 中浜 雅士・Reboul, Julien・亀井 謙一郎・北川 進・古川 修平
  • Organizer: 日本化学会第92 春季年会
  • Year and Date: 20120325-28
• [Presentation] Evaluation of Negative Photoresists on Phenotypes of Human Induced Pluripotent Stem Cells (hiPSCs) (2012)
  • Author(s): K. Kamei, Y. Hirai, Y. Makino, L. Liu, Q. Yuan, M. Yoshioka, Y. Chen, O. Tabata
  • Organizer: The IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2012-03-08
• [Presentation] Electrospun nanofibers for creating artificial cellular microenvironments for culturing mouse embryonic stem cells (2011)
  • Author(s): L. Liu, Q. Yuan, J. Shi, X. Li, D. Jung, L. Wang, K. Yamauchi, N. Nakatsuji, K. Kamei, Y. Chen
  • Organizer: 2nd Nanotoday Conference
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20111211-15
• [Presentation] Quantitative Cytological Feature Analysis by Microfluidic Image Cytometry Reveals Phenotypic Differences among Human Pluripotent Stem Cell Lines (2011)
  • Author(s): K. Kamei, M. Ohashi, N. A. Graham, Y. Chen, A. T. Clark, O. N. Witte, T. G. Graeber, A. D. Pyle and H.-R. Tseng
  • Organizer: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 20110000
• [Presentation] Quantitative Cytological Feature Analysis by Microfluidic Image Cytometry Reveals Phenotypic Differences among Human Pluripotent Stem Cell Lines (2011)
  • Author(s): K. Kamei, M. Ohashi, N. Graham, L. Liu, Q. Yuan, A. T. Clark, O.N. Witte, T. G. Graeber, A. D. Pyle, N. Nakatsuji, Y. Chen and H.-R. Tseng
  • Organizer: 9th International Society of Stem Cell Research (ISSCR) Annual Meeting
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20110000
• [Remarks]
  • URL: http://www.chen.icems.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://ken1kamei.wordpress.com/
• [Remarks] 亀井 謙一郎、古川 修平「ナノバイオテクノロジーが創出する学際融合研究の場: 6th Annual Symposium on Nanobiotechnology 」実験医学 , 31(3), 592-595 (2013)
• [Remarks] 亀井 謙一郎「ナノメディシン:ナノ粒子の臨床応用に向けて」実験医学, 30(12), 1928-1929 (2012)
• [Patent(Industrial Property Rights)] ファイバー・オン・ファイバーを用いた多能性幹細胞の3次元 (2013)
  • Inventor(s): 亀井 謙一郎、中辻 憲夫、劉 莉
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 特願 2013-117242
  • Filing Date: 2013-06-03
• [Patent(Industrial Property Rights)] 多能性幹細胞の培養方法及びそのための基材 (2012)
  • Inventor(s): 陳 勇、亀井 謙一郎、劉 莉
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 特願 2012-127580
  • Filing Date: 2012-06-04