2013 Fiscal Year Final Research Report
Method for culturing of unculturable phytoplasma by a hybrid-genome strategy
| Project/Area Number |
23681043
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KAKIZAWA shigeyuki 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (10588669)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | ゲノム / 細菌 |
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| Research Abstract |
A vector system for the whole genome cloning was established. A previously reported circular YAC (yeast artificial chromosome) vector was modified and used for genome cloning. Using this new system, high efficiency of positive clones was observed. A method to extract phytoplasma genome from infected plant tissues was also established. In addition, a multiplex-PCR system was established to confirm cloning of large genomic fragment. Strain-identification of phytoplasmas in infected host was also performed using the multiplex-PCR system.
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