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2013 Fiscal Year Final Research Report

Discovery of novel archaeal lineages and their ecophysiological functions in terrestrial subsurface environment

Research Project

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Project/Area Number 23681044
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Applied genomics
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

TAMAKI Hideyuki  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (00421842)

Project Period (FY) 2011-04-01 – 2014-03-31
Keywordsアーキア / 未知微生物 / 環境ゲノム / 培養 / 次世代シークエンス / 陸域地下圏 / メタン生成
Research Abstract

This study investigated phylogeny and ecophysiplogical functions of uncultured archaea and their related bacteria likely contributing to biogeochemical processes in terrestrial subsurface environments by polyphasic approaches including geochemical, microbiological and the advanced molecular ecological approaches. In particular, the present study discovered unidentified phylum or class level lineages in the domain Archaea in terrestrial subsurface environments such as glacial till, deep high temperature groundwater, deep oil & natural gas fields, etc. Single cell genome analysis provided genome fragments of some of the uncultured archaea and bacteria found in the terrestrial subsurface environments. Furthermore, we have succeeded in obtaining enrichment culture and pure culture of uncultured archaea from terrestrial subsurface environments and characterized their phylogenetic and ecophysiological functions.

  • Research Products

    (31 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (14 results) (of which Invited: 8 results) Book (1 results) Remarks (1 results)

  • [Journal Article] 無酸素環境におけるメタンの生成と消費サイクル2013

    • Author(s)
      鎌形洋一、玉木秀幸
    • Journal Title

      生物の科学遺伝

      Volume: 64 Pages: 586-590

    • Peer Reviewed
  • [Journal Article] 大量シークエンス情報解析時代の未知微生物探索2013

    • Author(s)
      玉木秀幸
    • Journal Title

      日本乳酸菌学会誌

      Volume: 24 Pages: 26-33

    • Peer Reviewed
  • [Journal Article] メタゲノム解析により見出された温泉環境に優占する未培養巨大鎌状細菌の硫黄代謝2013

    • Author(s)
      玉澤聡、玉木秀幸
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 71 Pages: 338-340

    • Peer Reviewed
  • [Journal Article] CO2 concentration dictates alternative methanogenic pathways in oil reservoir2013

    • Author(s)
      Mayumi D, Dolfing J, Sakata S, Maeda H, Miyagawa Y, Ikarashi M, Tamaki H, Takeuchi M, Nakatsu CH, Kamagata Y
    • Journal Title

      Nature Communications

      Volume: 4 Pages: 1998

    • DOI

      10.1038ncomms2998

    • Peer Reviewed
  • [Journal Article] Candidatus Methanogranum caenicola : a novel methanogen from the anaerobic digested sludge, and proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a methanogenic lineage of the class Thermoplasmata2013

    • Author(s)
      Iino T, Tamaki H, Tamazawa S, Ueno Y, Ohkuma M, Suzuki K, Igarashi Y, Haruta S
    • Journal Title

      Microbes and Environ

      Volume: 28 Pages: 244-250

    • Peer Reviewed
  • [Journal Article] Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei2013

    • Author(s)
      Katoh H, Tamaki H, Tokutake Y, Hanada S, Chohnan S
    • Journal Title

      J. Bioscience Bioengineering

      Volume: 115 Pages: 372-376

    • Peer Reviewed
  • [Journal Article] Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic thermophilic methanogen isolated from gas-associated formation water of a natural gas field in Japan2013

    • Author(s)
      Nakamura K, Takahashi A, Mori C, Tamaki H, Mochimaru H, Nakamura K, Takamizawa K and Kamagata Y
    • Journal Title

      Int. J. Syst. Evol. Microbiol

      Volume: 63 Pages: 715-722

    • Peer Reviewed
  • [Journal Article] 環境ゲノム情報時代の未知微生物探索研究2012

    • Author(s)
      玉木秀幸、鎌形洋一
    • Journal Title

      化学と生物

      Volume: 50 Pages: 730-741

    • Peer Reviewed
  • [Journal Article] Metagenomic and Biochemical Characterizations of Sulfur Oxidation Metabolism in Uncultured Large Sausage-Shaped Bacterium in Hot Spring Microbial Mats2012

    • Author(s)
      Tamazawa S, Takasaki K, Tamaki H, Kamagata Y, Hanada S
    • Journal Title

      PLoS One

      Volume: 7 Pages: e49794

    • Peer Reviewed
  • [Journal Article] Analysis of 16S rRNA amplicon sequencing options on the Roche/454 Next-Generation Titanium Sequencing platform2011

    • Author(s)
      Tamaki H, Wright CL, Li X, Lin Q, Hwang C, Wang S, Thimmapuram J, Kamagata Y & Liu WT
    • Journal Title

      PLoS One

      Volume: 6 Pages: e25263

    • Peer Reviewed
  • [Journal Article] A distinct freshwater-adapted subgroup of ANME-1 dominates active archaeal communities in terrestrial subsurfaces in Japan2011

    • Author(s)
      Takeuchi M, Yoshioka H, Seo Y, Tanabe S, Tamaki H, Kamagata Y, Takahashi HA, Igari S, Mayumi D and Sakata M
    • Journal Title

      Environ. Microbiol

      Volume: 13 Pages: 3206-3218

  • [Journal Article] A six well plate method : less laborious and effective method for cultivation of obligate anaerobic microorganisms2011

    • Author(s)
      Nakamura K, Tamaki H, Kang MS, Mochimaru H, Lee ST and Kamagata Y
    • Journal Title

      Microbes Environ

      Volume: 26 Pages: 301-306

  • [Journal Article] The genome of Syntrophorhabdus aromaticivorans strain UI provides new insights for syntrophic aromatic compound metabolism and electron flow

    • Author(s)
      Nobu M, Narihiro T, Tamaki H, Qiu YL, Sekiguchi Y, Woyke T, Goodwin L, Davenport KW, Kamagata Y, Liu WT
    • Journal Title

      Environmental Microbiology

      Volume: (受理済)

    • Peer Reviewed
  • [Journal Article] Halomonas sulfidaeris-dominated Communities Inhabit 1.8km-deep Saline Sandstone Reservoirs

    • Author(s)
      Dong Y, Kumar CG, Chia N, Kim PJ, Miller PA, Price ND, Cann I, Flynn T, Sanford R, Krapac IG, Locke RA, Hong P, Tamaki H, Liu WT, Hernandez AG, Wright CL, Mikel MA, Walker JL, Sivaguru M, Fried G, Yannarell AC, Mackie R, Fouke BW
    • Journal Title

      Environmental Microbiology

      Volume: (受理済)

    • Peer Reviewed
  • [Journal Article] Methyloceanibacter caenitepidi gen. nov., sp. nov., a novel facultatively methylotrophic bacterium isolated from marine sediments near the hydrothermal vent area

    • Author(s)
      Takeuchi M, Katayama T, Yamagishi T, Hanada S, Tamaki H, Kamagata Y, Ohshima K, Hattori M, Marumo K, Nedachi M, Maeda H, Suwa Y, Sakata S
    • Journal Title

      Int. J. Syst. Evol. Microbiol

      Volume: (受理済)

    • Peer Reviewed
  • [Presentation] 培養法で切拓く未知微生物の新機能2014

    • Author(s)
      玉木秀幸
    • Organizer
      日本農芸化学会2014大会
    • Place of Presentation
      明治大学(東京都)
    • Year and Date
      2014-03-30
    • Invited
  • [Presentation] 第三のドメイン「アーキア」:その世界的な研究動向と最新のトピック2013

    • Author(s)
      玉木秀幸
    • Organizer
      第29回日本微生物生態学会
    • Place of Presentation
      鹿児島大学(鹿児島県)
    • Year and Date
      2013-11-25
    • Invited
  • [Presentation] 分離培養を通して見える複合微生物生態系の世界2013

    • Author(s)
      玉木秀幸
    • Organizer
      第44回中部化学連合関係学協会支部連合秋季大会
    • Place of Presentation
      静岡大学(静岡県)
    • Year and Date
      2013-11-02
    • Invited
  • [Presentation] 環境オミックス情報解析時代の未知・未培養微生物の探索と資源化2013

    • Author(s)
      玉木秀幸
    • Organizer
      日本微生物資源学会第20回大会
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Year and Date
      2013-06-28
    • Invited
  • [Presentation] 関東平野の沖積層において新たに発見された陸域における微生物による嫌気的メタン酸化活動2012

    • Author(s)
      竹内美緒、吉岡秀佳、徐維那、田辺晋、玉木秀幸, ほか
    • Organizer
      第22回環境地質学シンポジウム
    • Place of Presentation
      つくば市(茨城県)
    • Year and Date
      2012-12-07
  • [Presentation] Microbial activities of methanogenesis and methane oxidation in terrestrial subsurface environment of the Kanto Plain in Japan2012

    • Author(s)
      吉岡秀佳、竹内美緒、田邊晋、高橋浩、玉木秀幸, ほか
    • Organizer
      American Geophysical Union's 45^<th> annual fall meeting
    • Year and Date
      2012-12-07
  • [Presentation] 大量シークエンス情報解析時代の未知微生物探索2012

    • Author(s)
      玉木秀幸
    • Organizer
      2012年度日本乳酸菌学会秋期セミナー
    • Place of Presentation
      明治大学(東京都)
    • Year and Date
      2012-11-30
    • Invited
  • [Presentation] Close Encounters of the Third Kind in Microbial Ecology2012

    • Author(s)
      玉木秀幸
    • Organizer
      イリノイ大学環境土木工学科分子生態学セミナー
    • Place of Presentation
      イリノイ(米国)
    • Year and Date
      2012-11-09
    • Invited
  • [Presentation] Diversity and activity of methanogenic microbial community in a high-temperature biodegraded oil field2012

    • Author(s)
      持丸華子、眞弓大介、吉岡秀佳、坂田将、玉木秀幸、鎌形洋一
    • Organizer
      日本微生物生態学会第28回大会
    • Place of Presentation
      豊橋技術科学大学(愛知県)
    • Year and Date
      2012-09-20
  • [Presentation] 高温高圧培養で切拓く陸域地下微生物群集の未知機能2012

    • Author(s)
      眞弓大介、坂田将、前田治男、宮川善洋、五十嵐雅之、玉木秀幸, ほか
    • Organizer
      日本微生物生態学会第28回大会シンポジウム
    • Place of Presentation
      豊橋技術科学大学(愛知県)
    • Year and Date
      2012-09-20
    • Invited
  • [Presentation] 第三種接近遭遇–Close Encounters of the Third Kind in Microbial Ecology-2012

    • Author(s)
      玉木秀幸
    • Organizer
      日本微生物生態学会第28回大会若手交流会
    • Place of Presentation
      豊橋技術科学大学(愛知県)
    • Year and Date
      2012-09-19
    • Invited
  • [Presentation] 深部地下油層環境における原油の生分解と生物的メタン生成2012

    • Author(s)
      持丸華子、吉岡秀佳、眞弓大介、坂田将、玉木秀幸、鎌形洋一
    • Organizer
      日本地球化学会
    • Place of Presentation
      九州大学(福岡県)
    • Year and Date
      2012-09-11
  • [Presentation] Methanogenic diversity and activity in a high-temperature biodegraded oil field2012

    • Author(s)
      持丸華子、眞弓大介、吉岡秀佳、坂田将、玉木秀幸、鎌形洋一
    • Organizer
      14th International Society for Microbial Ecology
    • Place of Presentation
      コペンハーゲン(デンマーク)
    • Year and Date
      2012-08-21
  • [Presentation] Impact of CO2 geosequestration on the methanogenic ecosystem in a high temperature petroleum reservoir2011

    • Author(s)
      眞弓大介、坂田将、前田治男、五十嵐雅之、宮川善洋、玉木秀幸, ほか
    • Organizer
      8^<th> International Symposium of Subsurface Microbiology
    • Place of Presentation
      Garmisch-Partenkirchen(ドイツ)
    • Year and Date
      2011-09-11
  • [Book] 環境と微生物の辞典2014

    • Author(s)
      玉木秀幸
    • Publisher
      朝倉出版
  • [Remarks] ①プレスリリース:CO2地中貯留がもたら す地下微生物生態系への影響を解明, 各種 新聞報道がなされた。

    • URL

      http://www.aist.go.jp/aist_j/press_release/pr2013/pr20130613/pr20130613.html

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Published: 2015-07-16  

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